Anti-GNAS antibody [7G6G5] (ab216129)
Key features and details
- Mouse monoclonal [7G6G5] to GNAS
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-GNAS antibody [7G6G5]
See all GNAS primary antibodies -
Description
Mouse monoclonal [7G6G5] to GNAS -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human G protein alpha aa 42-188. Expressed in E. Coli.
Database link: P63092 -
Positive control
- WB: Human G protein alpha (aa: 42-188) recombinant protein; Human G protein alpha (aa: 42-188)-hIgGFc transfected HEK293 cell lysate. ICC/IF: HeLa cells. FACS: MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
7G6G5 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 45 kDa.
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ICC/IF |
1/200 - 1/1000.
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Flow Cyt |
1/200 - 1/400.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 45 kDa. |
ICC/IF
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. |
Target
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Relevance
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The Gs protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Alternative splicing of downstream exons of the GNAS gene is observed, which results in different forms of the stimulatory G protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. -
Cellular localization
Cell Membrane -
Database links
- Entrez Gene: 2778 Human
- Omim: 139320 Human
- SwissProt: P63092 Human
- SwissProt: Q5JWF2 Human
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Alternative names
- Adenylate cyclase stimulating G alpha protein antibody
- AHO antibody
- Alternative gene product encoded by XL exon antibody
see all
Images
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Flow cytometric analysis of MCF7 cells labeling G protein alpha with ab216129 at 1/200 dilution (green). Negative control (red).
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Immunofluorescent analaysis of HeLa cells labeling G protein alpha with ab216129 at 1/200 dilution. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
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All lanes : Anti-GNAS antibody [7G6G5] (ab216129) at 1/500 dilution
Lane 1 : non-transfected HEK293 cell lysate
Lane 2 : Human G protein alpha (aa: 42-188)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 45 kDa -
Anti-GNAS antibody [7G6G5] (ab216129) at 1/500 dilution + Human G protein alpha (aa: 42-188) recombinant protein
Predicted band size: 45 kDaExpected MW is 42.8 kDa
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab216129 has not yet been referenced specifically in any publications.