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G0 Protein alpha protein (Tagged-His Tag) (ab91689)

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Overview

  • Product nameG0 Protein alpha protein (Tagged-His Tag)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 8-329 of Human G0 Protein alpha with N terminal His tag, 322 amino acids, 37kDa. UniProt ID = P09471.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 133 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesEERAALERSKAIEKNLKEDGISAAKDVKLLLLGAGESGKS TIVKQ MKIIHEDGFSGEDVKQYKPVVYSNTIQSLAAIV RAMDTLGIEYGD KERKADAKMVCDVVSRMEDTEPFSAE LLSAMMRLWGDSGIQECFN RSREYQLNDSAKYYLDSLD RIGAADYQPTEQDILRTRVKTTGIVE THFTFKNLHFRL FDVGGQRSERKKWIHCFEDVTAIIFCVALSGYD QVLHE DETTNRMHESLMLFDSICNNKFFIDTSIILFLNKKDLFGE KIKKSPLTICFPEYTGPNTYEDAAAYIQAQFESKNRSP NKEIYCH MTCATDT
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91689 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        DKFZp686O0962G alpha oG protein alpha 0
        GalphaoGNAOGNAO 1GNAO_HUMANGNAO1Go alphaGuanine nucleotide binding protein (G protein) alpha activating activity polypeptide OGuanine nucleotide binding protein alpha activating polypeptideGuanine nucleotide binding protein G(o) subunit alphaGuanine nucleotide-binding protein G(o) subunit alpha
      see all
  • FunctionGuanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(o) protein function is not clear. Stimulated by RGS14.
  • Sequence similaritiesBelongs to the G-alpha family. G(i/o/t/z) subfamily.

    • Target information above from: UniProt accession P09471 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    G0 Protein alpha protein (Tagged-His Tag) images

    • SDS-PAGE - G0 Protein alpha protein (Tagged-His Tag) (ab91689)
      SDS-PAGE - G0 Protein alpha protein (Tagged-His Tag) (ab91689)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for G0 Protein alpha protein (Tagged-His Tag) (ab91689)

    ab91689 has not yet been referenced specifically in any publications.

    Publishing research using ab91689 ? Please let us know so that we can cite the reference in this datasheet

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