Anti-G3BP antibody (ab123379)

Overview

• Product nameAnti-G3BP antibodySee all G3BP primary antibodies ...
• Description
  Rabbit polyclonal to G3BP
• Tested applicationsWB, IHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide: SSSPA, corresponding to a region around amino acids 230-234 of Human G3BP (Q13283).

  Run BLAST with Run BLAST with
• Positive control293 cell extract, Human breast carcinoma tissue

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferpH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
  Note: (without Mg2+ and Ca2+)
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA / Nucleotides

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • RNA Processing
  • Other

Applications

Target

• FunctionMay be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.
• Tissue specificityUbiquitous.
• Sequence similaritiesContains 1 NTF2 domain.
  Contains 1 RRM (RNA recognition motif) domain.
• DomainThe NTF2 domain mediates multimerization.
• Post-translational
  modificationsPhosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization.
  Arg-435 is dimethylated, probably to asymmetric dimethylarginine.
• Cellular localizationCytoplasm. Cytoplasm > cytosol. Cell membrane. Nucleus. Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells (By similarity). Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS.

Target information above from: UniProt accession Q13283 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 10146 Human
  • Omim: 608431 Human
  • SwissProt: Q13283 Human
  • Unigene: 587054 Human

• Alternative names
  ATP dependent DNA helicase VIII antibodyATP-dependent DNA helicase VIII antibodyG3BP antibody

  G3BP-1 antibodyG3bp1 antibodyG3BP1_HUMAN antibodyGAP binding protein antibodyGAP SH3 domain binding protein 1 antibodyGAP SH3 domain-binding protein 1 antibodyGTPase activating protein (SH3 domain) binding protein 1 antibodyhDH VIII antibodyMGC111040 antibodyRas GTPase activating protein binding protein 1 antibodyRas GTPase activating protein SH3 domain binding protein antibodyRas GTPase-activating protein-binding protein 1 antibodyRasGAP associated endoribonuclease G3BP antibody

  see all

Anti-G3BP antibody images

• 

  Western blot - Anti-G3BP antibody (ab123379)
  All lanes : Anti-G3BP antibody (ab123379) at 1/500 dilution
  
  Lane 1 : 293 cell extract with blocking peptide
  Lane 2 : 293 cell extract
  
  
  Predicted band size : 52 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G3BP antibody (ab123379)
  ab123379 at 1/50 dilution staining G3BP in Paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry. ab123379 preincubated with blocking peptide (right).