Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab123379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa). Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking over night.
IHC-P 1/50 - 1/100.

Target

  • FunctionMay be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesContains 1 NTF2 domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • DomainThe NTF2 domain mediates multimerization.
  • Post-translational
    modifications
    Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization.
    Arg-435 is dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localizationCytoplasm. Cytoplasm > cytosol. Cell membrane. Nucleus. Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells (By similarity). Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent DNA helicase VIII antibody
    • ATP-dependent DNA helicase VIII antibody
    • G3BP antibody
    • G3BP-1 antibody
    • G3bp1 antibody
    • G3BP1_HUMAN antibody
    • GAP binding protein antibody
    • GAP SH3 domain binding protein 1 antibody
    • GAP SH3 domain-binding protein 1 antibody
    • GTPase activating protein (SH3 domain) binding protein 1 antibody
    • hDH VIII antibody
    • Human DNA helicase VIII antibody
    • MGC111040 antibody
    • Ras GTPase activating protein binding protein 1 antibody
    • Ras GTPase activating protein SH3 domain binding protein antibody
    • Ras GTPase-activating protein-binding protein 1 antibody
    • RasGAP associated endoribonuclease G3BP antibody
    see all

Anti-G3BP antibody images

  • All lanes : Anti-G3BP antibody (ab123379) at 1/500 dilution

    Lane 1 : 293 cell extract with blocking peptide
    Lane 2 : 293 cell extract


    Predicted band size : 52 kDa
  • ab123379 at 1/50 dilution staining G3BP in Paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry. ab123379 preincubated with blocking peptide (right).

References for Anti-G3BP antibody (ab123379)

ab123379 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"