Anti-G3BP2 antibody (ab86135)
Key features and details
- Rabbit polyclonal to G3BP2
- Suitable for: ICC/IF, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-G3BP2 antibody
See all G3BP2 primary antibodies -
Description
Rabbit polyclonal to G3BP2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Orangutan -
Immunogen
Synthetic peptide corresponding to Human G3BP2 aa 250-350.
Database link: Q9UN86 -
Positive control
- ICC/IF: HeLa cells. WB: HeLa, MCF7, Jurkat, HepG2 and HEK293T whole cell lysate. IP: HEK293T whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 5 µg/ml.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 54 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
1/2000 - 1/10000. Predicted molecular weight: 54 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Probable scaffold protein that may be involved in mRNA transport. -
Sequence similarities
Contains 1 NTF2 domain.
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsArg-457 and Arg-468 are dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 9908 Human
- Entrez Gene: 23881 Mouse
- SwissProt: Q9UN86 Human
- SwissProt: P97379 Mouse
- SwissProt: Q5R9L3 Orangutan
- Unigene: 303676 Human
- Unigene: 290530 Mouse
- Unigene: 473827 Mouse
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Alternative names
- G3BP 2 antibody
- G3BP-2 antibody
- G3BP2 antibody
see all
Images
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ICC/IF image of ab86135 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86135, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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All lanes : Anti-G3BP2 antibody (ab86135) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : MCF7 whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lane 5 : HEK293T whole cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 54 kDa
Exposure time: 75 secondsDetection: Chemiluminescence.
Lysates prepared using NETN lysis buffer.
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G3BP2 was immunoprecipitated from 1 mg HEK293T whole cell lysate with ab86135 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab86135 at 0.04 µg/mL.
Lysates prepared using NETN lysis buffer.
Lane 1: ab86135 IP in HEK293T whole cell lysate.
Lane 2: Contol IgG in HEK293T whole cell lysate.
Detection: Chemiluminescence.
Exposure time: 1 second.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab86135 has been referenced in 15 publications.
- Nabeel-Shah S et al. SARS-CoV-2 nucleocapsid protein binds host mRNAs and attenuates stress granules to impair host stress response. iScience 25:103562 (2022). PubMed: 34901782
- Jia X et al. De novo variants in genes regulating stress granule assembly associate with neurodevelopmental disorders. Sci Adv 8:eabo7112 (2022). PubMed: 35977029
- Gao B et al. Inhibition of anti-viral stress granule formation by coronavirus endoribonuclease nsp15 ensures efficient virus replication. PLoS Pathog 17:e1008690 (2021). PubMed: 33635931
- Lei Y et al. O-GlcNAcylation of PFKFB3 is required for tumor cell proliferation under hypoxia. Oncogenesis 9:21 (2020). PubMed: 32060258
- Pla-Martín D et al. CLUH granules coordinate translation of mitochondrial proteins with mTORC1 signaling and mitophagy. EMBO J 39:e102731 (2020). PubMed: 32149416