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Western blot - GAB1 (phospho Y627) antibody (ab12863)
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Product Name
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GAB1 (phospho Y627) antibody
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See all GAB1 antibodies (9)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to GAB1 (phospho Y627)
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Immunogen
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Synthetic peptide (Human).
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Reacts with
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Ms
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Predicted to react
(species key)
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Hu
(Due to sequence homology)
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Specificity
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Does not cross react with Gab1 at tyrosines 242, 437, 472 or 476.
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000. Detects a band of approximately 115 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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NIH3T3 cells +/- PDGF.
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Cellular localization
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Cytoplasmic (probable).
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Research areas
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Cancer >> Signal transduction >> Other
Cancer >> Cell cycle >> Cell differentiation
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Cell Biology >> Apoptosis >> Intracellular >> Kinases
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Relevance
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Gab1 is a 115 kDa multiple docking protein that plays an essential role in cellular growth, transformation and apoptosis. Gab1 can be phosphorylated by multiple receptor tyrosine kinase (RTKs), including: insulin receptor (IR), platelet derived growth factor receptor beta] (PDGFRbeta]), hepatocyte growth factor/scatter factor receptor (HGFR/SFR or c Met), and epidermal growth factor receptor (EGF), as well as in response to cell cell adhesion. Gab1 is tyrosine phosphorylated on at least 16 sites, some of which serve as binding sites for phosphoatidylinositol 3 kinase (PI3K), Grb2, PLC gamma 1, Nck, and SHP2. Phosphorylation of Gab1 on tyrosines 627 and 659 is critical for its binding to SHP2, and for activation of the ERK/MAPK pathway in response to EGF.
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GAB1 (phospho Y627) antibody (ab12863) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: Dulbeco's PBS (without Mg2+ and Ca2+), 1mg/ml BSA. pH 7.3 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GAB1 (phospho Y627) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for GAB1 (phospho Y627) antibody images, references, products related to ab12863 and other tools.
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GAB1 (phospho Y627) antibody images:
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 Western blot - GAB1 (phospho Y627) antibody (ab12863)
Peptide Competition
Lysates prepared from NIH3T3 cells left untreated (1) or treated with PDGF (2-5). were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature and incubated with ab12863 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphosotyrosine containing peptide (4), the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method. The data show that only the peptide corresponding to ab12863 blocks the signal, verifying the specificity of the antibody.
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References for GAB1 (phospho Y627) antibody (ab12863)
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This product has been used in:
Mood K et al. Gab1 is required for cell cycle transition, cell proliferation, and transformation induced by an oncogenic met receptor. Mol Biol Cell 17:3717-28 (2006). PubMed: 16775003
If you publish research using ab12863 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to GAB1
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