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All lanes : Anti-GAB1 (phospho Y627) antibody (ab47771) at 1/500 dilution
Lane 1 : HuvEc cell extract, untreated.
Lane 2 : HuvEc cell extract treated with EGF (200ng/ml, 10min)
Predicted band size : 77 kDa
Observed band size : 115 kDa (why is the actual band size different from the predicted?)
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