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Anti-GAB1 (phospho Y627) antibody (ab47771)

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Overview

Product name

Anti-GAB1 (phospho Y627) antibody
See all GAB1 products (10) ...

Description

Rabbit polyclonal to GAB1 (phospho Y627)

Specificity

This antibody detects Gab1 only when phosphorylated at tyrosine 627.

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse

Immunogen

Synthesized phosphopeptide derived from human Gab1 around the phosphorylation site of tyrosine 627 (V-E-YP-L-D).

Positive control

HuvEc cell extract

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - GAB1 (phospho Y627) antibody (ab47771)Western blot - GAB1 (phospho Y627) antibody (ab47771) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab47771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 115 kDa (predicted molecular weight: 77 kDa).

  • ELISA

     ELISA: 1/10000

    ELISA: 1/10000

Target

Function

Probably involved in EGF and insulin receptor signaling.

Sequence similarities

Belongs to the GAB family.
Contains 1 PH domain.

Post-translational
modifications

Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains.

Target information above from: UniProt accessionQ13480 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Gab 1 antibody
  • Gab1 antibody
  • GAB1_HUMAN antibody
  • GRB 2 associated binder 1 antibody
  • GRB 2 associated binding protein 1 antibody
  • GRB2 associated binding protein 1 isoform a antibody
  • GRB2 associated binding protein 1 isoform b antibody
  • GRB2-associated binder 1 antibody
  • GRB2-associated-binding protein 1 antibody
  • Growth factor receptor bound protein 2-associated protein 1 antibody
see all

Anti-GAB1 (phospho Y627) antibody images:

  Western blot - GAB1 (phospho Y627) antibody (ab47771)

Western blot - GAB1 (phospho Y627) antibody (ab47771)

All lanes : Anti-GAB1 (phospho Y627) antibody (ab47771) at 1/500 dilution

Lane 1 : HuvEc cell extract, untreated.
Lane 2 : HuvEc cell extract treated with EGF (200ng/ml, 10min)


Predicted band size : 77 kDa
Observed band size : 115 kDa (why is the actual band size different from the predicted?)

References for Anti-GAB1 (phospho Y627) antibody (ab47771)

ab47771 has not yet been referenced specifically in any publications.

Publishing research using ab47771? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"