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Products:Cell Biology >> Apoptosis >> Intracellular >> Kinases
Overview
Product name
Anti-GAB1 (phospho Y627) antibody
See all GAB1 products (10) ...
Description
Rabbit polyclonal to GAB1 (phospho Y627)
Specificity
This antibody detects Gab1 only when phosphorylated at tyrosine 627.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthesized phosphopeptide derived from human Gab1 around the phosphorylation site of tyrosine 627 (V-E-YP-L-D).
Positive control
HuvEc cell extract
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Other
Cancer >> Cell cycle >> Cell differentiation
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Cell Biology >> Apoptosis >> Intracellular >> Kinases
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Western blot - GAB1 (phospho Y627) antibody (ab47771)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab47771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 115 kDa (predicted molecular weight: 77 kDa).
ELISA
ELISA: 1/10000ELISA: 1/10000
Target
Function
Probably involved in EGF and insulin receptor signaling.
Sequence similarities
Belongs to the GAB family.
Contains 1 PH domain.
Post-translational
modifications
Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains.
Target information above from: UniProt accessionQ13480
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Gab 1 antibody
- Gab1 antibody
- GAB1_HUMAN antibody
see all
Database links
- Entrez Gene: 2549 Human
- Entrez Gene: 14388 Mouse
- Omim: 604439 Human
- SwissProt: Q13480 Human
- SwissProt: Q9QYY0 Mouse
- Unigene: 618456 Human
- Unigene: 80720 Human
- Unigene: 277409 Mouse
Anti-GAB1 (phospho Y627) antibody images:
Western blot - GAB1 (phospho Y627) antibody (ab47771)
All lanes : Anti-GAB1 (phospho Y627) antibody (ab47771) at 1/500 dilution
Lane 1 : HuvEc cell extract, untreated.
Lane 2 : HuvEc cell extract treated with EGF (200ng/ml, 10min)
Predicted band size : 77 kDa
Observed band size : 115 kDa (why is the actual band size different from the predicted?)
References for Anti-GAB1 (phospho Y627) antibody (ab47771)
ab47771 has not yet been referenced specifically in any publications.
Publishing research using ab47771? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"