Feedback Form

Western blot - GAB1 (phospho Y627) antibody (ab47771)
Western blot - GAB1 (phospho Y627) antibody (ab47771)
(enlarge)


Product Name 

GAB1 (phospho Y627) antibody

 

See all GAB1 antibodies (9)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to GAB1 (phospho Y627)

Immunogen 

Synthesized phosphopeptide derived from human Gab1 around the phosphorylation site of tyrosine 627 (V-E-YP-L-D).

Reacts with 
(species key)

Hu, Ms

Specificity 

This antibody detects Gab1 only when phosphorylated at tyrosine 627.

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews

Submit an Abreview

Be the first to review this antibody and earn 220 Abpoints.


Application notes 
(see key)

Recommended dilutions
ELISA: 1/10000.
WB: 1/500 - 1/1000. Detects a band of approximately 115 kDa (predicted molecular weight: 77 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HuvEc cell extract

Cellular localization 

Cytoplasmic (probable).

Research areas 

Cancer >> Signal transduction >> Other
Cancer >> Cell cycle >> Cell differentiation
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Cell Biology >> Apoptosis >> Intracellular >> Kinases

Relevance 

Gab1 is a 115 kDa multiple docking protein that plays an essential role in cellular growth, transformation and apoptosis. Gab1 can be phosphorylated by multiple receptor tyrosine kinase (RTKs), including: insulin receptor (IR), platelet derived growth factor receptor beta] (PDGFRbeta]), hepatocyte growth factor/scatter factor receptor (HGFR/SFR or c Met), and epidermal growth factor receptor (EGF), as well as in response to cell cell adhesion. Gab1 is tyrosine phosphorylated on at least 16 sites, some of which serve as binding sites for phosphoatidylinositol 3 kinase (PI3K), Grb2, PLC gamma 1, Nck, and SHP2. Phosphorylation of Gab1 on tyrosines 627 and 659 is critical for its binding to SHP2, and for activation of the ERK/MAPK pathway in response to EGF.

 

Alternative names GAB1 (phospho Y627) antibody (ab47771)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

2549    (Human)
14388    (Mouse)

Omim

    

604439    (Human)

SwissProt

    

Q13480    (Human)
Q9QYY0    (Mouse)

Unigene

    

618456    (Human)
80720    (Human)
277409    (Mouse)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GAB1 (phospho Y627) antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for GAB1 (phospho Y627) antibody images, references, products related to ab47771 and other tools.

 

GAB1 (phospho Y627) antibody images:

Product image  Western blot - GAB1 (phospho Y627) antibody (ab47771)

Western blot - GAB1 (phospho Y627) antibody (ab47771)

All lanes : GAB1 (phospho Y627) antibody (ab47771) at 1/500 dilution

Lane 1 : HuvEc cell extract, untreated.
Lane 2 : HuvEc cell extract treated with EGF (200ng/ml, 10min)


Predicted band size : 77 kDa
Observed band size : 115 kDa (why is the actual band size different from the predicted?)

 
 

Search PubMed (MEDLINE) for references to GAB1

 

GAB1 (phospho Y627) antibody - more information

Customer reviews (feedback) regarding GAB1 (phospho Y627) antibody

Customer FAQs regarding GAB1 (phospho Y627) antibody

Protocols for GAB1 (phospho Y627) antibody

Price and availability of products related to GAB1 (phospho Y627) antibody

 

GAB1 (phospho Y627) antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Corresponding non-phospho antibody for GAB1 (phospho Y627) antibody

ab47513

  

Rabbit polyclonal to GAB1

 

Compatible Secondaries for GAB1 (phospho Y627) antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other GAB1 Antibodies

See all GAB1 antibodies (9)...

 

GAB1 (phospho Y627) antibody - other tools:

Contact Abcam with a Technical Enquiry about GAB1 (phospho Y627) antibody

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.