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Products:Cell Biology >> Apoptosis >> Intracellular >> Kinases
Overview
Product name
Anti-GAB1 (phospho Y659) antibody
See all GAB1 products (10) ...
Description
Rabbit polyclonal to GAB1 (phospho Y659)
Specificity
This antibody detects endogenous levels of GAB1 only when phosphorylated at tyrosine 659.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phosphopeptide derived from human GAB1 around the phosphorylation site of tyrosine 659 (VDYPVV).
Epitope
Human: Tyr659; Mouse: Tyr660; Rat: Tyr659
Positive control
NIH/3T3 cell extracts
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg+2 and Ca+2), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Other
Cancer >> Cell cycle >> Cell differentiation
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Cell Biology >> Apoptosis >> Intracellular >> Kinases
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Western blot - GAB1 (phospho Y659) antibody (ab59410)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
ELISA
ELISA: 1/5000ELISA: 1/5000
Target
Function
Probably involved in EGF and insulin receptor signaling.
Sequence similarities
Belongs to the GAB family.
Contains 1 PH domain.
Post-translational
modifications
Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains.
Target information above from: UniProt accessionQ13480
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Gab 1 antibody
- Gab1 antibody
- GAB1_HUMAN antibody
see all
Database links
- Entrez Gene: 2549 Human
- Entrez Gene: 14388 Mouse
- Entrez Gene: 361388 Rat
- Omim: 604439 Human
- SwissProt: Q13480 Human
- SwissProt: Q9QYY0 Mouse
- SwissProt: C6JUQ2 Rat
- Unigene: 618456 Human
see all
Anti-GAB1 (phospho Y659) antibody images:
Western blot - GAB1 (phospho Y659) antibody (ab59410)
All lanes : Anti-GAB1 (phospho Y659) antibody (ab59410) at 1/500 dilution
Lane 1 : NIH/3T3 cells,
treated with Insulin (0.01U/ml, 15mins)
Lane 2 : NIH/3T3 cells,
treated with Insulin (0.01U/ml, 15mins) with immunizing phosphopeptide
Predicted band size : 77 kDa
Observed band size : 77 kDa
References for Anti-GAB1 (phospho Y659) antibody (ab59410)
ab59410 has not yet been referenced specifically in any publications.
Publishing research using ab59410? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"