GAB2 antibody [EP369Y] (ab32365)

Product Name 

GAB2 antibody [EP369Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP369Y] to GAB2

Immunogen 

Synthetic peptide corresponding to residues in human Gab2

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

This antibody is predicted to detect the splice isoform 2 based on sequence homology.

Tested applications 
(see key)

Flow Cyt, ICC/IF, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/20.
ICC/IF: 1/50.

IP: 1/50.
WB: 1/500. Detects a band of approximately 97 kDa (predicted molecular weight: 74 kDa).

Is unsuitable for IHC.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

PC12 cells; K562 cells; MCF7 cells

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Signal transduction >> Other
Signal Transduction >> Adapters >> Cytoplasmic
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other

Relevance 

Gab2 (Grb2-associated binder-2) is an adaptor protein involved in numerous intracellular signaling pathways via its interaction with receptor tyrosine kinases (RTKs), growth factors and cytokines. Gab2 is a substrate of various RTKs, including EGFR, insulin receptor, cytokine receptors as well as B and T cell antigen receptors; Gab2 acts as a docking protein for several SH2-containing proteins. Upon tyrosine phosphorylation, Gab2 interacts with SHP2 tyrosine phosphatase and GRB2 adaptor protein PI3-kinase, CrkL and SHC.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP369Y

Isotype 

IgG

Purity 

Whole Cell Lysate

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

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GAB2 antibody [EP369Y] images:

References for GAB2 antibody [EP369Y] (ab32365)

  This product has been used in:

Pyarajan S et al. Interleukin-3 (IL-3)-induced c-fos Activation Is Modulated by Gab2-Calcineurin Interaction. J Biol Chem 283:23505-9 (2008). WB. PubMed: 18586679

If you publish research using ab32365 please let us know so that we can cite the reference on this datasheet.

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