![Western blot - GAB2 antibody [EP369Y] (ab32365) image](#ab32365WB.bmp)
![Immunofluorescence - GAB2 antibody [EP369Y] (ab32365) image](#ab32365ICC.bmp)
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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Overview
Product name
Anti-GAB2 antibody [EP369Y]
See all GAB2 products (9) ...
Description
Rabbit monoclonal [EP369Y] to GAB2
Specificity
This antibody is predicted to detect the splice isoform 2 based on sequence homology.
Tested applications
WB, ICC/IF, Flow Cyt, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to residues in human Gab2
Positive control
PC12 cells; K562 cells; MCF7 cells
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4
Concentration
Concentration information loading...
Purity
Whole Cell Lysate
Clonality
Monoclonal
Clone number
EP369Y
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Other
Signal Transduction >> Adapters >> Cytoplasmic
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500Detects a band of approximately 97 kDa (predicted molecular weight: 74 kDa).
ICC/IF: 1/50
Flow Cyt
Flow Cyt: 1/20Flow Cyt: 1/20
IP
IP: 1/50IP: 1/50
Application notes
Is unsuitable for or IHC.
Target
Relevance
Gab2 (Grb2-associated binder-2) is an adaptor protein involved in numerous intracellular signaling pathways via its interaction with receptor tyrosine kinases (RTKs), growth factors and cytokines. Gab2 is a substrate of various RTKs, including EGFR, insulin receptor, cytokine receptors as well as B and T cell antigen receptors; Gab2 acts as a docking protein for several SH2-containing proteins. Upon tyrosine phosphorylation, Gab2 interacts with SHP2 tyrosine phosphatase and GRB2 adaptor protein PI3-kinase, CrkL and SHC.
Cellular localization
Cytoplasm. Cell membrane
Alternative names
- GRB2 associated binder 2 pp100 antibody
- GAB 2 antibody
- Grb 2 associated binder 2 antibody
see all
Database links
- Entrez Gene: 9846 Human
- Entrez Gene: 14389 Mouse
- Entrez Gene: 84477 Rat
- Omim: 606203 Human
- SwissProt: Q9UQC2 Human
- SwissProt: Q9Z1S8 Mouse
- SwissProt: Q9EQH1 Rat
Anti-GAB2 antibody [EP369Y] images:
Western blot - GAB2 antibody [EP369Y] (ab32365)
![Western blot - GAB2 antibody [EP369Y] (ab32365)](/ps/datasheet/Images/32/ab32365/ab32365WB.bmp)
All lanes : Anti-GAB2 antibody [EP369Y] (ab32365) at 1/500 dilution
Lane 1 : K526 cell lysate
Lane 2 : MCF-7 cell lysate
Predicted band size : 74 kDa
Observed band size : 97 kDa (why is the actual band size different from the predicted?)
Immunofluorescence - GAB2 antibody [EP369Y] (ab32365)
![Immunofluorescence - GAB2 antibody [EP369Y] (ab32365)](/ps/datasheet/Images/32/ab32365/ab32365ICC.bmp)
Immunofluorescent staining of PC-12 cells
using ab32365 at 1/50 dilution
References for Anti-GAB2 antibody [EP369Y] (ab32365)
This product has been referenced in:
- Pyarajan Set al. Interleukin-3 (IL-3)-induced c-fos Activation Is Modulated by Gab2-Calcineurin Interaction. J Biol Chem 283:23505-9 (2008). WB.Read more (PubMed: 18586679) »
See 1 publication for this product
Publishing research using ab32365? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"