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Western blot - GAB2 antibody (ab62802)
(enlarge)
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Product Name
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GAB2 antibody
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See all GAB2 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to GAB2
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Immunogen
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Synthetic peptide: C-QVDKEKTQALQN, corresponding to internal sequence amino acids 645-656 of Human GAB2
BLAST 'C-QVDKEKTQALQN' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Dog
(Due to sequence homology)
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Specificity
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This antibody is expected to recognize both reported isoforms (NP_536739.1; NP_036428.1).
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Peptide ELISA: antibody detection limit dilution 1:64,000.
WB: Use at a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 74 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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K562 cell lysate.
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Cellular localization
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Cytoplasm. Cell membrane
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Research areas
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Cancer >> Signal transduction >> Other
Signal Transduction >> Adapters >> Cytoplasmic
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
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Relevance
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Gab2 (Grb2-associated binder-2) is an adaptor protein involved in numerous intracellular signaling pathways via its interaction with receptor tyrosine kinases (RTKs), growth factors and cytokines. Gab2 is a substrate of various RTKs, including EGFR, insulin receptor, cytokine receptors as well as B and T cell antigen receptors; Gab2 acts as a docking protein for several SH2-containing proteins. Upon tyrosine phosphorylation, Gab2 interacts with SHP2 tyrosine phosphatase and GRB2 adaptor protein PI3-kinase, CrkL and SHC.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GAB2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for GAB2 antibody images, references, products related to ab62802 and other tools.
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GAB2 antibody images:
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 Western blot - GAB2 antibody (ab62802)
GAB2 antibody (ab62802) at 1 µg/ml + K562 cell lysate (35µg protein in RIPA buffer).
Predicted band size : 74 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
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Search PubMed (MEDLINE) for references to GAB2
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GAB2 antibody - more information
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Customer reviews (feedback) regarding GAB2 antibody |
Customer FAQs regarding GAB2 antibody |
Protocols for GAB2 antibody |
Price and availability of products related to GAB2 antibody |
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GAB2 antibody - related products:
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GAB2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about GAB2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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