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Anti-GAB2 antibody (ab62802)

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Overview

Product name

Anti-GAB2 antibody
See all GAB2 products (9) ...

Description

Goat polyclonal to GAB2

Specificity

This antibody is expected to recognize both reported isoforms (NP_536739.1; NP_036428.1).

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Dog

Immunogen

Synthetic peptide: C-QVDKEKTQALQN, corresponding to internal sequence amino acids 645-656 of Human GAB2

C-QVDKEKTQ ALQN

Positive control

K562 cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - GAB2 antibody (ab62802)Western blot - GAB2 antibody (ab62802) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab62802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Peptide ELISA: antibody detection limit dilution 1:64,000.
WB: Use at a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 74 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Gab2 (Grb2-associated binder-2) is an adaptor protein involved in numerous intracellular signaling pathways via its interaction with receptor tyrosine kinases (RTKs), growth factors and cytokines. Gab2 is a substrate of various RTKs, including EGFR, insulin receptor, cytokine receptors as well as B and T cell antigen receptors; Gab2 acts as a docking protein for several SH2-containing proteins. Upon tyrosine phosphorylation, Gab2 interacts with SHP2 tyrosine phosphatase and GRB2 adaptor protein PI3-kinase, CrkL and SHC.

Cellular localization

Cytoplasm. Cell membrane

Alternative names

  • GRB2 associated binder 2 pp100 antibody
  • GAB 2 antibody
  • Grb 2 associated binder 2 antibody
  • GRB 2 associated binding protein 2 antibody
  • Grb2 associated binder 2 antibody
  • GRB2 associated binding protein 2 antibody
  • Growth factor receptor bound protein 2 associated protein 2 antibody
  • KIAA0571 antibody
  • p97 antibody
  • PH domain containing adaptor molecule p97 antibody
  • pp100 antibody
see all

Anti-GAB2 antibody images:

  Western blot - GAB2 antibody (ab62802)

Western blot - GAB2 antibody (ab62802)

Anti-GAB2 antibody (ab62802) at 1 µg/ml + K562 cell lysate (35µg protein in RIPA buffer).

Predicted band size : 74 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)


Primary incubation was 1 hour. Detected by chemiluminescence.

References for Anti-GAB2 antibody (ab62802)

ab62802 has not yet been referenced specifically in any publications.

Publishing research using ab62802? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"