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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Overview
Product name
Anti-GAB2 antibody
See all GAB2 products (9) ...
Description
Goat polyclonal to GAB2
Specificity
This antibody is expected to recognize both reported isoforms (NP_536739.1; NP_036428.1).
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Dog
Immunogen
Synthetic peptide: C-QVDKEKTQALQN, corresponding to internal sequence amino acids 645-656 of Human GAB2
C-QVDKEKTQ ALQN
Positive control
K562 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Other
Signal Transduction >> Adapters >> Cytoplasmic
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
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Western blot - GAB2 antibody (ab62802)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab62802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
Peptide ELISA: antibody detection limit dilution 1:64,000.
WB: Use at a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 74 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Gab2 (Grb2-associated binder-2) is an adaptor protein involved in numerous intracellular signaling pathways via its interaction with receptor tyrosine kinases (RTKs), growth factors and cytokines. Gab2 is a substrate of various RTKs, including EGFR, insulin receptor, cytokine receptors as well as B and T cell antigen receptors; Gab2 acts as a docking protein for several SH2-containing proteins. Upon tyrosine phosphorylation, Gab2 interacts with SHP2 tyrosine phosphatase and GRB2 adaptor protein PI3-kinase, CrkL and SHC.
Cellular localization
Cytoplasm. Cell membrane
Alternative names
- GRB2 associated binder 2 pp100 antibody
- GAB 2 antibody
- Grb 2 associated binder 2 antibody
see all
Database links
- Entrez Gene: 9846 Human
- Entrez Gene: 14389 Mouse
- Entrez Gene: 84477 Rat
- Omim: 606203 Human
- SwissProt: Q9UQC2 Human
- SwissProt: Q9Z1S8 Mouse
- SwissProt: Q9EQH1 Rat
Anti-GAB2 antibody images:
Western blot - GAB2 antibody (ab62802)
Anti-GAB2 antibody (ab62802) at 1 µg/ml + K562 cell lysate (35µg protein in RIPA buffer).
Predicted band size : 74 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
References for Anti-GAB2 antibody (ab62802)
ab62802 has not yet been referenced specifically in any publications.
Publishing research using ab62802? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"