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Western blot - GAB2 antibody (ab62802)
Western blot - GAB2 antibody (ab62802)
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Product Name 

GAB2 antibody

 

See all GAB2 antibodies (7)...

Product type 

Primary antibodies

Description 

Goat polyclonal to GAB2

Immunogen 

Synthetic peptide: C-QVDKEKTQALQN, corresponding to internal sequence amino acids 645-656 of Human GAB2
BLAST 'C-QVDKEKTQALQN' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Rat, Dog
(Due to sequence homology)

Specificity 

This antibody is expected to recognize both reported isoforms (NP_536739.1; NP_036428.1).

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
Peptide ELISA: antibody detection limit dilution 1:64,000.
WB: Use at a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 74 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

K562 cell lysate.

Cellular localization 

Cytoplasm. Cell membrane

Research areas 

Cancer >> Signal transduction >> Other
Signal Transduction >> Adapters >> Cytoplasmic
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other

Relevance 

Gab2 (Grb2-associated binder-2) is an adaptor protein involved in numerous intracellular signaling pathways via its interaction with receptor tyrosine kinases (RTKs), growth factors and cytokines. Gab2 is a substrate of various RTKs, including EGFR, insulin receptor, cytokine receptors as well as B and T cell antigen receptors; Gab2 acts as a docking protein for several SH2-containing proteins. Upon tyrosine phosphorylation, Gab2 interacts with SHP2 tyrosine phosphatase and GRB2 adaptor protein PI3-kinase, CrkL and SHC.

 

Alternative names GAB2 antibody (ab62802)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

9846    (Human)
14389    (Mouse)
84477    (Rat)

Omim

    

606203    (Human)

SwissProt

    

Q9UQC2    (Human)
Q9Z1S8    (Mouse)
Q9EQH1    (Rat)

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GAB2 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for GAB2 antibody images, references, products related to ab62802 and other tools.

 

GAB2 antibody images:

Product image  Western blot - GAB2 antibody (ab62802)

Western blot - GAB2 antibody (ab62802)

GAB2 antibody (ab62802) at 1 µg/ml + K562 cell lysate (35µg protein in RIPA buffer).

Predicted band size : 74 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)


Primary incubation was 1 hour. Detected by chemiluminescence.

 
 

Search PubMed (MEDLINE) for references to GAB2

 

GAB2 antibody - more information

Customer reviews (feedback) regarding GAB2 antibody

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Price and availability of products related to GAB2 antibody

 

GAB2 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for GAB2 antibody

ab7911

  

K-562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

 

Compatible Secondaries for GAB2 antibody

ab6566

  

Cy5 ® antibody

ab6739

  

Texas Red ® antibody

ab6740

  

Biotin antibody

ab6741

  

HRP antibody

ab6742

  

Alkaline Phosphatase antibody

ab6881

  

FITC antibody

ab7004

  

Phycoerythrin antibody

ab27245

  

10nm Gold antibody

ab27246

  

10nm Gold antibody

ab27247

  

15nm Gold antibody

 

Other GAB2 Antibodies

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GAB2 antibody - other tools:

Contact Abcam with a Technical Enquiry about GAB2 antibody

 

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