Anti-GAB2 (phospho Y643) antibody (ab78262)
Key features and details
- Rabbit polyclonal to GAB2 (phospho Y643)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GAB2 (phospho Y643) antibody
See all GAB2 primary antibodies -
Description
Rabbit polyclonal to GAB2 (phospho Y643) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human GAB2 (phospho Y643).
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Positive control
- Extracts from Jurkat cells. Human brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab78262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
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IHC-P |
1/50 - 1/100.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa). |
IHC-P
1/50 - 1/100. |
Target
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Function
Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis. -
Sequence similarities
Belongs to the GAB family.
Contains 1 PH domain. -
Domain
The SH3-binding motifs mediate interaction with SHC1 and GRB2.
The PH domain mediates phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate binding. -
Post-translational
modificationsPhosphorylated on tyrosine residue(s) by the thrombopoietin receptor (TPOR), stem cell factor receptor (SCFR), and T-cell and B-cell antigen receptors, gp130, IL-2R and IL-3R (By similarity). Phosphorylated upon stimulation of TNFRSF11A/RANK by TNFSF11/RANKL (By similarity). Phosphorylated upon EGF stimulation. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.
Dephosphorylated by PTPN11. -
Cellular localization
Cytoplasm. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 9846 Human
- Entrez Gene: 14389 Mouse
- Entrez Gene: 84477 Rat
- Omim: 606203 Human
- SwissProt: Q9UQC2 Human
- SwissProt: Q9Z1S8 Mouse
- SwissProt: Q9EQH1 Rat
- Unigene: 429434 Human
see all -
Alternative names
- GAB 2 antibody
- Gab2 antibody
- GAB2_HUMAN antibody
see all
Images
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All lanes : Anti-GAB2 (phospho Y643) antibody (ab78262) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells, treated
with IFN (2500U/ML, 30mins)
Lane 2 : Extracts from Jurkat cells, treated
with IFN (2500U/ML, 30mins) plus 5µg immunizing peptide
Lysates/proteins at 5 µg per lane.
Predicted band size: 74 kDa
Observed band size: 74 kDa
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab78262 at a 1/50 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab78262 has been referenced in 1 publication.
- Yim JH et al. Phosphoprotein profiles of candidate markers for early cellular responses to low-dose ?-radiation in normal human fibroblast cells. J Radiat Res 58:329-340 (2017). WB ; Human . PubMed: 28122968