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Anti-GABA A Receptor alpha 1 antibody (ab33299)

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Overview

Product name

Anti-GABA A Receptor alpha 1 antibody
See all GABA A Receptor alpha 1 products (6) ...

Description

Rabbit polyclonal to GABA A Receptor alpha 1

Specificity

This antibody is specific for the GABA A Receptor a1 subunit.

Tested applications

IHC-P, WB, IP, IHC-Frmore details

Cross reactivity

Reacts with

Mouse, Rat, Chicken, Human

Predicted to work with

Cow, Cynomolgus Monkey

Immunogen

Synthetic peptide: QPSQDELKDNTTVFT-C, corresponding to N terminal amino acids 1-15 of the mature Rat GABA A Receptor alpha 1 containing a cysteine at the C terminus for linkage to a carrier protein.

QPSQDELKDN TTVFT-C

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

WB and IP: Rat brain microsomal tissue extract

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 30% Glycerol, 150mM Sodium chloride, 100mM Tris glycine. pH 7.4

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Neuroscience >> Neurotransmission >> Receptors / Channels >> Ligand-Gated Ion Channels >> GABA Receptors
Neuroscience >> Neurotransmitter >> Amino Acids >> GABA

  • Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 1 antibody (ab33299)Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 1 antibody (ab33299) image (enlarge)

Applications

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Our Abpromise guarantee covers the use of ab33299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at Gamma aminobutyric acid type A (GABA A) receptors. GABA A receptors are ligand gated chloride ion channels. These receptors are believed to be pentameric hetero oligomers, for which 6 subunits are currently known (alpha, beta, gamma, delta, epsilon and rho). GABA A receptors mediate the majority of fast inhibitor synaptic transmissions in the brain. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA A receptor.

Cellular localization

Membrane; multi pass membrane protein.

Alternative names

  • EJM antibody
  • Gaba receptor alpha 1 polypeptide antibody
  • GABA(A) receptor subunit alpha 1 antibody
  • GABRA 1 antibody
  • GABRA1 antibody
  • Gamma aminobutyric acid (GABA) A receptor alpha 1 antibody
  • Gamma aminobutyric acid A receptor alpha 1 antibody
  • Gamma aminobutyric acid receptor subunit alpha 1 antibody
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Database links

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Anti-GABA A Receptor alpha 1 antibody images:

  Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 1 antibody (ab33299)

Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 1 antibody (ab33299)

ab33299 staining GABA A Receptor alpha 1 in human retinal tissue by Immunohistochemistry (Frozen sections).

Tissue was fixed with 4% paraformaldehyde, blocked with 10% donkey serum for 20 minutes at 24°C, then incubated with ab33299 at a 1/100 dilution for 16 hours at 4°C. The secondary antibody used was an Alexa-Fluor 488 (green) conjugated donkey anti-rabbit polyclonal, used at a 1/1000 dilution. Counterstained with Hoechst 33258 (blue).

IPL - inner plexiform layer
INL - inner nuclear layer
OPL - outer plexiform layer
ONL - outer nuclear layer
INS - inner nuclear segments

Image courtesy of an anonymous Abreview.

See Abreview

References for Anti-GABA A Receptor alpha 1 antibody (ab33299)

This product has been referenced in:

  • Daniel Cet al. Adenosine-to-inosine RNA editing affects trafficking of the gamma-aminobutyric acid type A (GABA(A)) receptor. J Biol Chem 286:2031-40 (2011). WB; Mouse.Read more (PubMed: 21030585) »
  • Lindstrom SHet al. Retinal input to efferent target amacrine cells in the avian retina. Vis Neurosci 27:103-18 (2010). IHC-P; Chicken.Read more (PubMed: 20650017) »

See all 2 publications for this product

Publishing research using ab33299? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"