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Products:Neuroscience >> Neurotransmitter >> Amino Acids >> GABA
Overview
Product name
Anti-GABA A Receptor alpha 1 antibody
See all GABA A Receptor alpha 1 products (6) ...
Description
Rabbit polyclonal to GABA A Receptor alpha 1
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, a wide range of other species
Immunogen
Synthetic peptide derived from human GABA A Receptor alpha 1 protein.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurotransmission >> Receptors / Channels >> Ligand-Gated Ion Channels >> GABA Receptors
Neuroscience >> Neurotransmitter >> Amino Acids >> GABA
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Western blot - GABA A Receptor alpha 1 antibody (ab65269)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab65269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution.
WB: 1/500 - 1/5000.Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
Target
Relevance
GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at Gamma aminobutyric acid type A (GABA A) receptors. GABA A receptors are ligand gated chloride ion channels. These receptors are believed to be pentameric hetero oligomers, for which 6 subunits are currently known (alpha, beta, gamma, delta, epsilon and rho). GABA A receptors mediate the majority of fast inhibitor synaptic transmissions in the brain. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA A receptor.
Cellular localization
Membrane; multi pass membrane protein.
Alternative names
- EJM antibody
- Gaba receptor alpha 1 polypeptide antibody
- GABA(A) receptor antibody
see all
Database links
- Entrez Gene: 2554 Human
- Entrez Gene: 14394 Mouse
- Entrez Gene: 29705 Rat
- Omim: 137160 Human
- SwissProt: P14867 Human
- SwissProt: P18504 Mouse
- SwissProt: P62813 Rat
- Unigene: 175934 Human
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Anti-GABA A Receptor alpha 1 antibody images:
Western blot - GABA A Receptor alpha 1 antibody (ab65269)
Anti-GABA A Receptor alpha 1 antibody (ab65269) at 1/2000 dilution + Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 52 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
References for Anti-GABA A Receptor alpha 1 antibody (ab65269)
This product has been referenced in:
- Yeung LYet al. Localization of 5-HT1A and 5-HT2A positive cells in the brainstems of control age-matched and Alzheimer individuals. Age (Dordr) 32:483-95 (2010). IHC-P; Human.Read more (PubMed: 20508993) »
See 1 publication for this product
Publishing research using ab65269? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"