Anti-GABA A Receptor gamma 2/GABRG2 antibody (ab87328)
Key features and details
- Rabbit polyclonal to GABA A Receptor gamma 2/GABRG2
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GABA A Receptor gamma 2/GABRG2 antibody
See all GABA A Receptor gamma 2/GABRG2 primary antibodies -
Description
Rabbit polyclonal to GABA A Receptor gamma 2/GABRG2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following tissue lysates: human brain; mouse brain; human spinal cord; mouse spinal cord.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab87328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 54 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 54 kDa). |
Target
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Function
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. -
Involvement in disease
Defects in GABRG2 are the cause of childhood absence epilepsy type 2 (ECA2) [MIM:607681]. ECA2 is a subtype of idiopathic generalized epilepsy (IGE) characterized by an onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures develop. Some individuals manifest ECA2 occurring in combination with febrile convulsions.
Defects in GABRG2 are the cause of familial febrile convulsions type 8 (FEB8) [MIM:611277]. A febrile convulsion is defined as a seizure event in infancy or childhood, usually occurring between 6 months and 6 years of age, associated with fever but without any evidence of intracranial infection or defined pathologic or traumatic cause. Febrile convulsions affect 5-12% of infants and children up to 6 years of age. There is epidemiological evidence that febrile seizures are associated with subsequent afebrile and unprovoked seizures in 2% to 7% of patients.
Defects in GABRG2 are the cause of generalized epilepsy with febrile seizures plus type 3 (GEFS+3) [MIM:604233]. Generalized epilepsy with febrile seizures-plus refers to a rare autosomal dominant, familial condition with incomplete penetrance and large intrafamilial variability. Patients display febrile seizures persisting sometimes beyond the age of 6 years and/or a variety of afebrile seizure types. GEFS+ is a disease combining febrile seizures, generalized seizures often precipitated by fever at age 6 years or more, and partial seizures, with a variable degree of severity.
Defects in GABRG2 are a cause of severe myoclonic epilepsy in infancy (SMEI) [MIM:607208]; also called Dravet syndrome. SMEI is a rare disorder characterized by generalized tonic, clonic, and tonic-clonic seizures that are initially induced by fever and begin during the first year of life. Later, patients also manifest other seizure types, including absence, myoclonic, and simple and complex partial seizures. Psychomotor development delay is observed around the second year of life. SMEI is considered to be the most severe phenotype within the spectrum of generalized epilepsies with febrile seizures-plus. -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRG2 sub-subfamily. -
Post-translational
modificationsPalmitoylated by ZDHHC3/GODZ; which may affect presynaptic clustering and/or cell surface stability. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 282240 Cow
- Entrez Gene: 2566 Human
- Entrez Gene: 14406 Mouse
- Entrez Gene: 29709 Rat
- Omim: 137164 Human
- SwissProt: P22300 Cow
- SwissProt: P18507 Human
- SwissProt: P22723 Mouse
see all -
Alternative names
- CAE 2 antibody
- CAE2 antibody
- ECA 2 antibody
see all
Images
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All lanes : Anti-GABA A Receptor gamma 2/GABRG2 antibody (ab87328)
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa, 42 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutesWe hypothesize that the 51 kDa band represents the mature form of GABA A Receptor gamma 2/GABRG2.
Abcam welcomes customer feedback.
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All lanes : Anti-GABA A Receptor gamma 2/GABRG2 antibody (ab87328) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 45 kDa, 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab87328 has been referenced in 9 publications.
- Anagnostopoulos G et al. An obesogenic feedforward loop involving PPARγ, acyl-CoA binding protein and GABAA receptor. Cell Death Dis 13:356 (2022). PubMed: 35436993
- Li X et al. Neocortex- and hippocampus-specific deletion of Gabrg2 causes temperature-dependent seizures in mice. Cell Death Dis 12:553 (2021). PubMed: 34050134
- Bian Z et al. Mechanisms Underlying the Action of Ziziphi Spinosae Semen in the Treatment of Insomnia: A Study Involving Network Pharmacology and Experimental Validation. Front Pharmacol 12:752211 (2021). PubMed: 35002696
- Huo J et al. ALG13 participates in epileptogenesis via regulation of GABAA receptors in mouse models. Cell Death Discov 6:87 (2020). PubMed: 33014431
- Wang M et al. Schisandrin B exerts hypnotic effects in PCPA-treated rats by increasing hypothalamic 5-HT and ?-aminobutyric acid levels. Exp Ther Med 20:142 (2020). PubMed: 33093880
- Zheng J et al. Interneuron Accumulation of Phosphorylated tau Impairs Adult Hippocampal Neurogenesis by Suppressing GABAergic Transmission. Cell Stem Cell 26:331-345.e6 (2020). PubMed: 31978364
- Speigel IA et al. Chronic Calcineurin Inhibition via Cyclosporine A Impairs Visuospatial Learning After Isoflurane Anesthesia. Anesth Analg 129:192-203 (2019). PubMed: 31082969
- Chen M et al. APP modulates KCC2 expression and function in hippocampal GABAergic inhibition. Elife 6:N/A (2017). WB ; Mouse . PubMed: 28054918
- Bates RC et al. Reduced CHRNA7 expression in C3H mice is associated with increases in hippocampal parvalbumin and glutamate decarboxylase-67 (GAD67) as well as altered levels of GABA(A) receptor subunits. Neuroscience 273:52-64 (2014). PubMed: 24836856