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- Secondary antibodies
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Product name
Anti-GABA Transporter 1 / GAT 1 antibody
See all GABA Transporter 1 / GAT 1 products (4) ...
Description
Rabbit polyclonal to GABA Transporter 1 / GAT 1
Specificity
Reacts specifically with a 72 kDa protein.
Tested applications
IHC-P, IHC-Fr, WB, IHC-FoFrmore details
Cross reactivity
Reacts with
Mouse, Rat, Chicken
Predicted to work with
Human
Immunogen
20 residue C-terminal synthetic peptide (Rat).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole Serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurotransmitter >> Transporters >> GABA
Neuroscience >> Neurotransmitter >> Amino Acids >> GABA
Applications
Show applications key
Our Abpromise guarantee covers the use of ab426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
IHC-P: 1/50 - 1/500.IHC-P: 1/50 - 1/500.
IHC-Fr: 1/50 - 1/500.
WB: Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 67 kDa).
IHC-FoFr: Use at an assay dependent concentration.
Target
Relevance
GABA Transporter 1/GAT1 is a gamma-amino butyric acid (GABA) transporter. After its release from interneurons in the CNS, the major inhibitory neurotransmitter GABA is taken up by GABA transporters (GATs). The predominant neuronal GABA transporter GAT1 is localized in GABAergic axons and nerve terminals, where it is thought to influence GABAergic synaptic transmission by removing GABA from the synaptic cleft.
Cellular localization
Cell Membrane
Alternative names
- GABATHG antibody
- GABATR antibody
- GABT 1 antibody
see all
Database links
- Entrez Gene: 415960 Chicken
- Entrez Gene: 6529 Human
- Entrez Gene: 232333 Mouse
- Entrez Gene: 79212 Rat
- Omim: 137165 Human
- SwissProt: P30531 Human
- SwissProt: P31648 Mouse
- SwissProt: P23978 Rat
see all
Anti-GABA Transporter 1 / GAT 1 antibody images:
Western blot - GABA Transporter 1 / GAT 1 antibody (ab426)
All lanes : Anti-GABA Transporter 1 / GAT 1 antibody (ab426) at 1 µg/ml
Lane 1 : Rat Hippocampus Tissue Lysate
Lane 2 : Spinal Cord (Rat) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 67 kDa
Observed band size : 74 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
GABA Transporter 1/ GAT 1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Immunohistochemistry (Frozen sections) - GABA Transporter 1 / GAT 1 antibody (ab426)
ab426 staining the GABA transporter 1 in Mouse retinal tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/2000 in PBST) for 12 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal (1/1000) was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Kevin Jin
References for Anti-GABA Transporter 1 / GAT 1 antibody (ab426)
This product has been referenced in:
- Martínez-Cerdeño V et al. Embryonic MGE precursor cells grafted into adult rat striatum integrate and ameliorate motor symptoms in 6-OHDA-lesioned rats. Cell Stem Cell 6:238-50 (2010). IHC-FrFl; Rat.Read more (PubMed: 20207227) »
- François J et al. Selective reorganization of GABAergic transmission in neonatal ventral hippocampal-lesioned rats. Int J Neuropsychopharmacol 12:1097-110 (2009). IHC-FoFr; Rat.Read more (PubMed: 19267957) »
See all 7 publications for this product
Publishing research using ab426? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"