RabMAb

Anti-GABARAP antibody [EPR4806] (ab109299)

Overview

  • Product nameAnti-GABARAP antibody [EPR4806]
    See all GABARAP primary antibodies
  • Description
    Rabbit monoclonal [EPR4806] to GABARAP
  • Tested applicationsICC, WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Rat, Human

    Does not react with

    Mouse
  • Immunogen

    A synthetic peptide corresponding to residues in Human GABARAP

  • Positive control
    • SH-SY5Y cell lysate, Human fetal brain tissue lysate, Flag-GABARAP, Human heart tissue, Human brain tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/50 - 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at an assay dependent dilution.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionMay play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton.
    • Tissue specificityHeart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
    • Sequence similaritiesBelongs to the MAP1 LC3 family.
    • Cellular localizationEndomembrane system. Cytoplasm > cytoskeleton. Golgi apparatus membrane. Largely associated with intracellular membrane structures including the Golgi apparatus and post-synaptic cisternae. Colocalizes with microtubules.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATG8A antibody
      • FLC 3B antibody
      • FLC3B antibody
      • FLJ25768 antibody
      • GABA(A) receptor associated protein antibody
      • GABA(A) receptor-associated protein antibody
      • GABARAP a antibody
      • GABARAP antibody
      • Gamma aminobutyric acid receptor associated protein antibody
      • Gamma-aminobutyric acid receptor-associated protein antibody
      • GBRAP_HUMAN antibody
      • MGC120154 antibody
      • MGC120155 antibody
      • MM 46 antibody
      • MM46 antibody
      see all

    Anti-GABARAP antibody [EPR4806] images

    • All lanes : Anti-GABARAP antibody [EPR4806] (ab109299) at 1/1000 dilution

      Lane 1 : Fetal brain tissue lysate at 10 µg
      Lane 2 : SH-SY5Y cell lysate at 10 µg
      Lane 3 : Flag-GABARAP


      Predicted band size : 14 kDa
      Observed band size : 17 kDa (why is the actual band size different from the predicted?)
    • ab109299, at 1/100 dilution, staining GABARAP in Human heart tissue by immunohistochemistry.
    • ab109299, at 1/100 dilution, staining GABARAP in Human brain tissue by immunohistochemistry.

    References for Anti-GABARAP antibody [EPR4806] (ab109299)

    ab109299 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: High pH
    Sample Human Tissue sections (Tendon)
    Specification Tendon
    Permeabilization No
    Fixative 10% buffered formalin
    Username

    Abcam user community

    Verified customer

    Submitted Oct 22 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (SH-SY5Y cells)
    Specification SH-SY5Y cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Dr. George Allen

    Verified customer

    Submitted Sep 26 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"