Recombinant
RabMAb

Anti-GABARAP antibody [EPR4806] (ab109299)

Overview

  • Product name
    Anti-GABARAP antibody [EPR4806]
    See all GABARAP primary antibodies
  • Description
    Rabbit monoclonal [EPR4806] to GABARAP
  • Tested applications
    Suitable for: ICC, WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human GABARAP

  • Positive control
    • SH-SY5Y cell lysate, Human fetal brain tissue lysate, Flag-GABARAP, Human heart tissue, Human brain tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/50 - 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
    • Tissue specificity
      Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
    • Sequence similarities
      Belongs to the ATG8 family.
    • Post-translational
      modifications
      The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II.
    • Cellular localization
      Endomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome. Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity).
    • Information by UniProt
    • Database links
    • Alternative names
      • ATG8A antibody
      • FLC 3B antibody
      • FLC3B antibody
      • FLJ25768 antibody
      • GABA type A receptor associated protein antibody
      • GABA(A) receptor associated protein antibody
      • GABA(A) receptor-associated protein antibody
      • GABARAP a antibody
      • GABARAP antibody
      • Gamma aminobutyric acid receptor associated protein antibody
      • Gamma-aminobutyric acid receptor-associated protein antibody
      • GBRAP_HUMAN antibody
      • MGC120154 antibody
      • MGC120155 antibody
      • MM 46 antibody
      • MM46 antibody
      see all

    Images

    • All lanes : Anti-GABARAP antibody [EPR4806] (ab109299) at 1/1000 dilution

      Lane 1 : Human fetal brain tissue lysate at 10 µg
      Lane 2 : SH-SY5Y cell lysate at 10 µg
      Lane 3 : Flag-GABARAP


      Predicted band size : 14 kDa
      Observed band size : 17 kDa (why is the actual band size different from the predicted?)
    • ab109299, at 1/100 dilution, staining GABARAP in Human heart tissue by immunohistochemistry.
    • ab109299, at 1/100 dilution, staining GABARAP in Human brain tissue by immunohistochemistry.

    References

    ab109299 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: High pH
    Sample
    Human Tissue sections (Tendon)
    Specification
    Tendon
    Permeabilization
    No
    Fixative
    10% buffered formalin
    Username

    Abcam user community

    Verified customer

    Submitted Oct 22 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (SH-SY5Y cells)
    Specification
    SH-SY5Y cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Dr. George Allen

    Verified customer

    Submitted Sep 26 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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