The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at an assay dependent concentration.
Application notesIs unsuitable for Flow Cyt.
FunctionMay play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton.
Tissue specificityHeart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the MAP1 LC3 family.
Cellular localizationEndomembrane system. Cytoplasm > cytoskeleton. Golgi apparatus membrane. Largely associated with intracellular membrane structures including the Golgi apparatus and post-synaptic cisternae. Colocalizes with microtubules.