RabMAb

Anti-GABARAP antibody [EPR4806] (ab109299)

Overview

  • Product nameAnti-GABARAP antibody [EPR4806]
    See all GABARAP primary antibodies
  • Description
    Rabbit monoclonal [EPR4806] to GABARAP
  • Tested applicationsICC, WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human GABARAP

  • Positive control
    • SH-SY5Y cell lysate, Human fetal brain tissue lysate, Flag-GABARAP, Human heart tissue, Human brain tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab109299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/50 - 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at an assay dependent dilution.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionMay play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton.
    • Tissue specificityHeart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
    • Sequence similaritiesBelongs to the MAP1 LC3 family.
    • Cellular localizationEndomembrane system. Cytoplasm > cytoskeleton. Golgi apparatus membrane. Largely associated with intracellular membrane structures including the Golgi apparatus and post-synaptic cisternae. Colocalizes with microtubules.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATG8A antibody
      • FLC 3B antibody
      • FLC3B antibody
      • FLJ25768 antibody
      • GABA(A) receptor associated protein antibody
      • GABA(A) receptor-associated protein antibody
      • GABARAP a antibody
      • GABARAP antibody
      • Gamma aminobutyric acid receptor associated protein antibody
      • Gamma-aminobutyric acid receptor-associated protein antibody
      • GBRAP_HUMAN antibody
      • MGC120154 antibody
      • MGC120155 antibody
      • MM 46 antibody
      • MM46 antibody
      see all

    Anti-GABARAP antibody [EPR4806] images

    • All lanes : Anti-GABARAP antibody [EPR4806] (ab109299) at 1/1000 dilution

      Lane 1 : Fetal brain tissue lysate at 10 µg
      Lane 2 : SH-SY5Y cell lysate at 10 µg
      Lane 3 : Flag-GABARAP


      Predicted band size : 14 kDa
      Observed band size : 17 kDa (why is the actual band size different from the predicted?)
    • ab109299, at 1/100 dilution, staining GABARAP in Human heart tissue by immunohistochemistry.
    • ab109299, at 1/100 dilution, staining GABARAP in Human brain tissue by immunohistochemistry.

    References for Anti-GABARAP antibody [EPR4806] (ab109299)

    ab109299 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: High pH
    Sample Human Tissue sections (Tendon)
    Specification Tendon
    Permeabilization No
    Fixative 10% buffered formalin
    Username

    Abcam user community

    Verified customer

    Submitted Oct 22 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (SH-SY5Y cells)
    Specification SH-SY5Y cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Dr. George Allen

    Verified customer

    Submitted Sep 26 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"