Anti-GABARAP antibody - N-terminal (ab228504)
Key features and details
- Rabbit polyclonal to GABARAP - N-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GABARAP antibody - N-terminal
See all GABARAP primary antibodies -
Description
Rabbit polyclonal to GABARAP - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rabbit, Cow -
Immunogen
Synthetic peptide corresponding to Human GABARAP aa 1-100 (N terminal) (Cysteine residue).
Database link: O95166 -
Positive control
- WB: HEK-293 and Odora cell lysates. ICC/IF: Human white blood cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7 -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/200 - 1/1000.
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WB |
1/200 - 1/1000. Predicted molecular weight: 13 kDa.
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Notes |
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ICC/IF
1/200 - 1/1000. |
WB
1/200 - 1/1000. Predicted molecular weight: 13 kDa. |
Target
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Function
Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. -
Tissue specificity
Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. -
Sequence similarities
Belongs to the ATG8 family. -
Post-translational
modificationsThe precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II. -
Cellular localization
Endomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome. Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity). - Information by UniProt
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Database links
- Entrez Gene: 327715 Cow
- Entrez Gene: 11337 Human
- Entrez Gene: 56486 Mouse
- Entrez Gene: 100008883 Rabbit
- Omim: 605125 Human
- SwissProt: Q9GJW7 Cow
- SwissProt: O95166 Human
- SwissProt: Q9DCD6 Mouse
see all -
Alternative names
- ATG8A antibody
- FLC 3B antibody
- FLC3B antibody
see all
Images
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Human white blood cells stained for GABARAP (red) using ab228504 at 1/200 dilution in ICC/IF.
MHC class staining is shown (green). The nuclear counterstain is Hoechst Dye (blue).
The merged image is shown.
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Anti-GABARAP antibody - N-terminal (ab228504) at 1/100 dilution + HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Developed using the ECL technique.
Predicted band size: 13 kDa -
Anti-GABARAP antibody - N-terminal (ab228504) at 1/100 dilution + Odora (human olfactory cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 13 kDa -
Human white blood cells stained for GABARAP (red) using ab228504 incubated for 1 hour at room temperature at 1/200 dilution in ICC/IF.
MHC class II staining is shown (green). Nuclear counterstain is Hoechst Dye (blue).
The merged image is shown in panel C.
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Human white blood cells stained for GABARAP (red) using ab228504 at 1/200 dilution in ICC/IF.
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Human white blood cells stained for GABARAP (red) using ab228504 at 1/200 dilution in ICC/IF.
MHC class staining is shown (green). The nuclear counterstain is Hoechst Dye (blue).
The merged image is shown.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab228504 has not yet been referenced specifically in any publications.