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Products:Neuroscience >> Neurotransmission >> Intracellular Signaling >> Adapters
Overview
Product name
Anti-GABARAP antibody
See all GABARAP products (5) ...
Description
Rabbit polyclonal to GABARAP
Tested applications
IHC-P, IHC-Fr, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide as a part of human, rat, mouse GABARAP conjugated to immunogenic carrier protein.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
-
Immunocytochemistry/ Immunofluorescence-GABARAP antibody(ab62471)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab62471 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
IHC-P: 1/300 - 1/3000.IHC-P: 1/300 - 1/3000.
IHC-Fr
IHC-Fr: 1/300 - 1/3000.IHC-Fr: 1/300 - 1/3000.
ICC/IF: Use a concentration of 5 µg/ml
Target
Function
May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton.
Tissue specificity
Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
Sequence similarities
Belongs to the MAP1 LC3 family.
Cellular localization
Endomembrane system. Cytoplasm > cytoskeleton. Golgi apparatus membrane. Largely associated with intracellular membrane structures including the Golgi apparatus and post-synaptic cisternae. Colocalizes with microtubules.
Target information above from: UniProt accessionO95166
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ATG8A antibody
- FLC 3B antibody
- FLC3B antibody
see all
Database links
- Entrez Gene: 11337 Human
- Entrez Gene: 56486 Mouse
- Entrez Gene: 58974 Rat
- Omim: 605125 Human
- SwissProt: O95166 Human
- SwissProt: Q9DCD6 Mouse
- SwissProt: P60517 Rat
- Unigene: 647421 Human
see all
Anti-GABARAP antibody images:
Immunocytochemistry/ Immunofluorescence-GABARAP antibody(ab62471)
ICC/IF image of ab62471 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62471, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-GABARAP antibody (ab62471)
ab62471 has not yet been referenced specifically in any publications.
Publishing research using ab62471? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"