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Anti-GABARAPL2 antibody (ab82900)

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Overview

Product name

Anti-GABARAPL2 antibody
See all GABARAPL2 products (6) ...

Description

Rabbit polyclonal to GABARAPL2

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Cow, Dog, Zebrafish

Immunogen

Synthetic peptide corresponding to a region within the N terminal amino acids 2-51 (KWMFKEDHSL EHRCVESAKI RAKYPDRVPV IVEKVSGSQI VDIDKRKYLV) of Human GABARAPL2 (NP_009216).

KWMFKEDHSL EHRCVESAKI RAKYPDRVPV IVEKVSGSQI VDIDKRKYLV

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

293T cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Signal transduction >> Autophagy
Signal Transduction >> Protein Trafficking >> Golgi Proteins
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation

  • Western blot - GABARAPL2 antibody (ab82900)Western blot - GABARAPL2 antibody (ab82900) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab82900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA titre using peptide based assay 1/62500.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 13 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

GABARAPL2 is involved in intra Golgi traffic and modulates intra Golgi transport through coupling between NSF activity and SNAREs activation. GABARAPL2 first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1.

Cellular localization

Golgi Apparatus

Alternative names

  • ATG8 antibody
  • FLC3A antibody
  • GATE16 antibody
  • GEF2 antibody

Database links

see all

Anti-GABARAPL2 antibody images:

  Western blot - GABARAPL2 antibody (ab82900)

Western blot - GABARAPL2 antibody (ab82900)

Anti-GABARAPL2 antibody (ab82900) at 1 µg/ml + 293T cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 13 kDa
Observed band size : 20 kDa (why is the actual band size different from the predicted?)


Gel concentration 10-20%

References for Anti-GABARAPL2 antibody (ab82900)

ab82900 has not yet been referenced specifically in any publications.

Publishing research using ab82900? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"