Anti-GABPB2/Nuclear respiratory factor 2 antibody (ab88746)
Key features and details
- Mouse polyclonal to Nuclear respiratory factor 2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GABPB2/Nuclear respiratory factor 2 antibody -
Description
Mouse polyclonal to Nuclear respiratory factor 2 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein within Human GABPB2/Nuclear respiratory factor 2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.
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IHC-P |
Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. |
IHC-P
Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene. -
Sequence similarities
Contains 5 ANK repeats. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2553 Human
- Entrez Gene: 499883 Rat
- Omim: 600610 Human
- SwissProt: Q06547 Human
- Unigene: 654350 Human
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Alternative names
- BABPB2 antibody
- E4TF1 antibody
- E4TF1-47 antibody
see all
Images
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Anti-GABPB2/Nuclear respiratory factor 2 antibody (ab88746) at 1 µg/ml + PC-12 cell lysate at 50 µg
Predicted band size: 42 kDa -
Anti-GABPB2/Nuclear respiratory factor 2 antibody (ab88746) at 1 µg/ml + HeLa nuclear extracts at 50 µg
Predicted band size: 42 kDa -
Anti-GABPB2/Nuclear respiratory factor 2 antibody (ab88746) at 1 µg/ml + Jurkat cell lysate at 50 µg
Predicted band size: 42 kDa -
All lanes : Anti-GABPB2/Nuclear respiratory factor 2 antibody (ab88746) at 1 µg/ml
Lane 1 : lysate from transfected 293T cells
Lane 2 : lysate from non-transfected 293T cells
Lysates/proteins at 25 µg per lane.
Predicted band size: 42 kDa -
ab88746 at 3 µg/ml staining Nuclear respiratory factor 2 in formalin-fixed, paraffin-embedded Human endometrium by immunohistochemistry.
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ab88746 at 10 µg/ml staining Nuclear respiratory factor 2 in PFA-fixed, permeabilised HeLa cells by immunofluorescence.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab88746 has been referenced in 2 publications.
- Hwang JH et al. TAZ links exercise to mitochondrial biogenesis via mitochondrial transcription factor A. Nat Commun 13:653 (2022). PubMed: 35115527
- Gallart-Palau X et al. Extracellular vesicles are rapidly purified from human plasma by PRotein Organic Solvent PRecipitation (PROSPR). Sci Rep 5:14664 (2015). PubMed: 26419333