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Products:Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Overview
Product name
Anti-GABPB2 antibody
See all GABPB2 products (4) ...
Description
Mouse polyclonal to GABPB2
Tested applications
WB, IHC-P, ICC/IF, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Full length Human protein (NP_005245.2)
Positive control
WB: PC-12 or Jurkat cell lysate; Hela nuclear extracts. IHC: endometrium. ICC/IF: HeLa cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1 x PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab88746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: Use at a concentration of 10 µg/ml.
IHC-P: Use at a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining. Microwave method recommended.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene.
Sequence similarities
Contains 5 ANK repeats.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ06547
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BABPB2 antibody
- E4TF1 antibody
- E4TF1-47 antibody
see all
Database links
- Entrez Gene: 2553 Human
- Entrez Gene: 14391 Mouse
- Entrez Gene: 364738 Rat
- Omim: 600610 Human
- SwissProt: Q06547 Human
- SwissProt: Q00420 Mouse
- SwissProt: Q4KM28 Rat
- Unigene: 654350 Human
see all
Anti-GABPB2 antibody images:
Western blot - GABPB2 antibody (ab88746)
Anti-GABPB2 antibody (ab88746) at 1 µg/ml + PC-12 cell lysate at 50 µg
Predicted band size : 42 kDa
Western blot - GABPB2 antibody (ab88746)
Anti-GABPB2 antibody (ab88746) at 1 µg/ml + HeLa nuclear extracts at 50 µg
Predicted band size : 42 kDa
Western blot - GABPB2 antibody (ab88746)
Anti-GABPB2 antibody (ab88746) at 1 µg/ml + Jurkat cell lysate at 50 µg
Predicted band size : 42 kDa
Western blot - GABPB2 antibody (ab88746)
All lanes : Anti-GABPB2 antibody (ab88746) at 1 µg/ml
Lane 1 : lysate from transfected 293T cells
Lane 2 : lysate from non-transfected 293T cells
Lysates/proteins at 25 µg per lane.
Predicted band size : 42 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GABPB2 antibody (ab88746)
ab88746 at 3 µg/ml staining GABPB2 in formalin-fixed, paraffin-embedded Human endometrium by immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - GABPB2 antibody (ab88746)
ab88746 at 10 µg/ml staining GABPB2 in PFA-fixed, permeabilised HeLa cells by immunofluorescence.
References for Anti-GABPB2 antibody (ab88746)
ab88746 has not yet been referenced specifically in any publications.
Publishing research using ab88746? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"