![Immunohistochemistry (PFA fixed) - GAD65 antibody [GAD-6] - Neuronal Marker (ab26113) image](#ab26113_1.jpg)
![Western blot - GAD65 antibody [GAD-6] - Neuronal Marker (ab26113) image](#GAD65-Primary-antibodies-ab26113-2.jpg)
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Products:Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
Overview
Product name
Anti-GAD65 antibody [GAD-6] - Neuronal Marker
See all GAD65 products (10) ...
Description
Mouse monoclonal [GAD-6] to GAD65 - Neuronal Marker
Specificity
The GAD-6 monoclonal antibody ab26113 recognizes only GAD65 and has no cross reactivity with GAD67 on western blots of mouse brain or purified recombinant GAD67 and GAD65. ab26113 recognizes GAD65 primarily in nerve terminals in immunohistochemical analysis of brain sections (Kaufman et al, 1991).
Tested applications
IHC-P, WB, IHC-Fr, IHC-FoFrmore details
Cross reactivity
Reacts with
Mouse, Rat
Predicted to work with
all Mammals
Immunogen
Full length protein (Rat)
Epitope
ab26113 recognizes an epitope near the C-terminus of GAD65
Positive control
mouse brain
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
GAD-6
Isotype
IgG2a
Research areas
Neuroscience >> Neurology process >> Metabolism
Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
Applications
Show applications key
Our Abpromise guarantee covers the use of ab26113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution. (PubMed: 18467596)
WB: 1/1000 - 1/5000.Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
IHC-Fr
IHC-Fr: 1/1000 - 1/5000.IHC-Fr: 1/1000 - 1/5000.
IHC-FoFr: Use at an assay dependent concentration. (PubMed: 20207227)
Target
Function
Catalyzes the production of GABA.
Sequence similarities
Belongs to the group II decarboxylase family.
Post-translational
modifications
Phosphorylated; which does not affect kinetic parameters or subcellular location.
Palmitoylated; which is required for presynaptic clustering.
Cellular localization
Cytoplasm > cytosol. Cytoplasmic vesicle. Cell junction > synapse > presynaptic cell membrane. Golgi apparatus membrane. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.
Target information above from: UniProt accessionQ05329
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 65 kDa glutamic acid decarboxylase antibody
- DCE 2 antibody
- DCE 2 antibody
see all
Database links
- Entrez Gene: 14417 Mouse
- Entrez Gene: 24380 Rat
- SwissProt: P48320 Mouse
- SwissProt: Q548L4 Mouse
- SwissProt: Q05683 Rat
- Unigene: 4784 Mouse
- Unigene: 29951 Rat
Anti-GAD65 antibody [GAD-6] - Neuronal Marker images:
Immunohistochemistry (PFA fixed) - GAD65 antibody [GAD-6] - Neuronal Marker (ab26113)
![Immunohistochemistry (PFA fixed) - GAD65 antibody [GAD-6] - Neuronal Marker (ab26113)](/ps/datasheet/Images/26/ab26113/ab26113_1.jpg)
Mouse monoclonal [GAD-6] to GAD65 staining GAD65-containing nerve terminals around cell bodies of Purkinje cells in the rat cerebellar cortex.
Western blot - GAD65 antibody [GAD-6] - Neuronal Marker (ab26113)
![Western blot - GAD65 antibody [GAD-6] - Neuronal Marker (ab26113)](/ps/datasheet/images/26/ab26113/GAD65-Primary-antibodies-ab26113-2.jpg)
All lanes : Anti-GAD65 antibody [GAD-6] - Neuronal Marker (ab26113) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 65 kDa
Observed band size : 65 kDa
Additional bands at : 57 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
References for Anti-GAD65 antibody [GAD-6] - Neuronal Marker (ab26113)
This product has been referenced in:
- Elizalde Net al. Sustained stress-induced changes in mice as a model for chronic depression. Psychopharmacology (Berl) 210:393-406 (2010). WB; Mouse.Read more (PubMed: 20401750) »
- Martínez-Cerdeño Vet al. Embryonic MGE precursor cells grafted into adult rat striatum integrate and ameliorate motor symptoms in 6-OHDA-lesioned rats. Cell Stem Cell 6:238-50 (2010). IHC-FoFr; Rat.Read more (PubMed: 20207227) »
See all 9 publications for this product
Publishing research using ab26113? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"