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Products:Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
Overview
Product name
Anti-GAD65 antibody
See all GAD65 products (10) ...
Description
Goat polyclonal to GAD65
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Dog, Chimpanzee
Immunogen
Synthetic peptide: C-TLEDNEERMSRLSK, corresponding to Internal sequence amino acids 515-528 of Human GAD65
C-TLEDNEER MSRLSK
Positive control
Human Brain lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Metabolism
Neuroscience >> Neurotransmitter >> Amino Acids >> Glutamate
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Western blot - GAD65 antibody (ab31278)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/32000ELISA: 1/32000
WB: Use a concentration of 0.1 - 0.3 µg/ml.Predicted molecular weight: 65 kDa.
Target
Function
Catalyzes the production of GABA.
Sequence similarities
Belongs to the group II decarboxylase family.
Post-translational
modifications
Phosphorylated; which does not affect kinetic parameters or subcellular location.
Palmitoylated; which is required for presynaptic clustering.
Cellular localization
Cytoplasm > cytosol. Cytoplasmic vesicle. Cell junction > synapse > presynaptic cell membrane. Golgi apparatus membrane. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.
Target information above from: UniProt accessionQ05329
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 65 kDa glutamic acid decarboxylase antibody
- DCE 2 antibody
- DCE2 antibody
see all
Database links
- Entrez Gene: 466026 Chimpanzee
- Entrez Gene: 487107 Dog
- Entrez Gene: 2572 Human
- Entrez Gene: 14417 Mouse
- Entrez Gene: 24380 Rat
- Omim: 138275 Human
- SwissProt: Q4PRC2 Dog
- SwissProt: Q05329 Human
see all
Anti-GAD65 antibody images:
Western blot - GAD65 antibody (ab31278)
Anti-GAD65 antibody (ab31278) at 0.1 µg/ml + Human Brain lysate (35µg protein in RIPA buffer)
Predicted band size : 65 kDa
Observed band size : 26,65,70 kDa (why is the actual band size different from the predicted?)
All bands were blocked by the immunising peptide.
Primary incubation was 1 hour. Detected by chemiluminescence.
References for Anti-GAD65 antibody (ab31278)
ab31278 has not yet been referenced specifically in any publications.
Publishing research using ab31278? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"