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Anti-GAD67 antibody [K-87] - Neuronal Marker (ab26116)

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Overview

Product name

Anti-GAD67 antibody [K-87] - Neuronal Marker
See all GAD67 products (9) ...

Description

Mouse monoclonal [K-87] to GAD67 - Neuronal Marker

Tested applications

ICC/IF, Flow Cyt, IHC-P, WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide: RFRRTETDFSNLFARDLLPA, corresponding to amino acids 87-106 of Human GAD67

RFRRTETDFSNLFARDLLPA

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

In Western Blot, this antibody gave a positive signal in Mouse and Rat Brain Tissue Lysates. GAD67 has been thought to be primarily located in the nerve cell body, but using this new K-87 mAb, GAD67 can now also be detected in dendrites and axons.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Ascites

Primary antibody notes

ab26116 mouse monoclonal [K-87] to GAD67 specically recognizes GAD67 and has no cross reactivity with GAD65; this has been shown on western blots of mouse brain or purified recombinant GAD67 and GAD65. In immunohistochemical analysis of brain sections, the K-87 monoclonal recognizes GAD67 in nerve cell bodies and has an enhanced ability to detect GAD67 in dendrites and axon buttons compared to the original anti-GAD67 K-2 polyclonal antibody (see Kaufman et al, 1991).

Clonality

Monoclonal

Clone number

K-87

Isotype

IgG1

Light chain type

kappa

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Amino acid metabolism
Immunology >> Immune System Diseases >> Autoimmune
Signal Transduction >> Metabolism >> Amino Acids

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GAD67 antibody [K-87] - Neuronal Marker (ab26116)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GAD67 antibody [K-87] - Neuronal Marker (ab26116) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Anti-GAD67 antibody [K-87] - Neuronal Marker (ab26116)Immunocytochemistry/ Immunofluorescence - Anti-GAD67 antibody [K-87] - Neuronal Marker (ab26116) image (enlarge)

  • Flow Cytometry - GAD67 antibody [K-87] - Neuronal Marker (ab26116)Flow Cytometry - GAD67 antibody [K-87] - Neuronal Marker (ab26116) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab26116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    ICC/IF

     ICC/IF: Use a concentrat...Read more →

    ICC/IF: Use a concentration of 10 µg/ml.

  • ShowHide1 Image

    Flow Cyt

      See more...Read more →

    Flow Cyt: Use 1µg for 106 cells. (methanol fixed cells)

  • ShowHide1 Image

    IHC-P

     IHC-P: 1/1000 - 1/10000....Read more →

    IHC-P: 1/1000 - 1/10000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.An antigen retrieval step is necessary (e.g. incubating the sections at 90 degrees C in a 50mM citrate buffer). Cell body labeling is optimized when Triton is omitted from the tissue processing. Axon terminal labeling is substantially increased (and cell body labeling decreased) when Triton is included (see Soghomonian et al, 1998).

  • ShowHide1 Image

    WB

     WB: 1/1000 - 1/5000. Det...Read more →

    WB: 1/1000 - 1/5000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).

Target

Function

Catalyzes the production of GABA.

Tissue specificity

Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.

Involvement in disease

Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture.

Sequence similarities

Belongs to the group II decarboxylase family.

Target information above from: UniProt accessionQ99259 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 67 kDa glutamic acid decarboxylase antibody
  • DCE1 antibody
  • DCE1_HUMAN antibody
  • EC 4.1.1.15 antibody
  • FLJ45882 antibody
  • GAD 1 antibody
  • GAD 67 antibody
  • GAD antibody
  • GAD-67 antibody
  • GAD1 antibody
  • Glutamate decarboxylase 1 (brain 67kD) antibody
  • Glutamate decarboxylase 1 antibody
  • Glutamate decarboxylase 1 brain 67kD antibody
  • Glutamate decarboxylase 1 brain 67kDa antibody
  • Glutamate decarboxylase 67 kDa isoform antibody
  • OTTHUMP00000041055 antibody
  • SCP antibody
see all

Database links

see all

Anti-GAD67 antibody [K-87] - Neuronal Marker images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GAD67 antibody [K-87] - Neuronal Marker (ab26116)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GAD67 antibody [K-87] - Neuronal Marker (ab26116)

ab26116, at a 1/2000 dilution, staining of fixed mouse cerebellum sections that underwent an antigen retrieval step.

  Immunocytochemistry/ Immunofluorescence - Anti-GAD67 antibody [K-87] - Neuronal Marker (ab26116)

Immunocytochemistry/ Immunofluorescence - Anti-GAD67 antibody [K-87] - Neuronal Marker (ab26116)

ICC/IF image of ab26116 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26116, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  Flow Cytometry - GAD67 antibody [K-87] - Neuronal Marker (ab26116)

Flow Cytometry - GAD67 antibody [K-87] - Neuronal Marker (ab26116)

Overlay histogram showing PC12 cells stained with ab26116 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26116, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

  Western blot - GAD67 antibody [K-87] - Neuronal Marker (ab26116)

Western blot - GAD67 antibody [K-87] - Neuronal Marker (ab26116)

All lanes : Anti-GAD67 antibody [K-87] - Neuronal Marker (ab26116) at 5 µg/ml

Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 67 kDa
Observed band size : 67 kDa


Exposure time : 12 minutes

References for Anti-GAD67 antibody [K-87] - Neuronal Marker (ab26116)

This product has been referenced in:

  • Chan O  et al. Increased GABAergic tone in the ventromedial hypothalamus contributes to suppression of counterregulatory responses after antecedent hypoglycemia. Diabetes 57:1363-70 (2008). WB; Rat.Read more (PubMed: 18375441) »
  • Martin DL & Barke KE Are GAD65 and GAD67 associated with specific pools of GABA in brain? Perspect Dev Neurobiol 5:119-29 (1998).Read more (PubMed: 9777630) »

See all 3 publications for this product

Publishing research using ab26116? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"