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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-GAD67 antibody
See all GAD67 products (9) ...
Description
Goat polyclonal to GAD67
Specificity
ab80589 is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2 (isoform GAD65).
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Cow, Cat, Dog, Pig, Chimpanzee, Orangutan
Immunogen
Synthetic peptide: C-PDSPQRREKLHK, corresponding to internal sequence amino acids 526 - 537 of Human GAD67 (NP_000808.2)
C-PDSPQRRE KLHK
Positive control
Mouse brain lysate, Rat brain lysate and Human brain (cerebellum) lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab80589 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Immune System Diseases >> Autoimmune
Signal Transduction >> Metabolism >> Amino Acids
Applications
Show applications key
Our Abpromise guarantee covers the use of ab80589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 1.5 µg/ml.Predicted molecular weight: 67 kDa.
Target
Function
Catalyzes the production of GABA.
Tissue specificity
Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
Involvement in disease
Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture.
Sequence similarities
Belongs to the group II decarboxylase family.
Target information above from: UniProt accessionQ99259
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 67 kDa glutamic acid decarboxylase antibody
- DCE1 antibody
- DCE1_HUMAN antibody
see all
Database links
- Entrez Gene: 517552 Cow
- Entrez Gene: 2571 Human
- Entrez Gene: 14415 Mouse
- Entrez Gene: 396928 Pig
- Entrez Gene: 24379 Rat
- Omim: 605363 Human
- SwissProt: Q0VCA1 Cow
- SwissProt: A0PA85 Dog
see all
Anti-GAD67 antibody images:
Western blot - GAD67 antibody (ab80589)
Anti-GAD67 antibody (ab80589) at 1 µg/ml + Mouse Brain lysate (in RIPA buffer) at 35 µg
Predicted band size : 67 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Western blot - GAD67 antibody (ab80589)
Anti-GAD67 antibody (ab80589) at 0.5 µg/ml + Human brain (cerebral cortex) lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 67 kDa
Observed band size : 67 kDa
References for Anti-GAD67 antibody (ab80589)
ab80589 has not yet been referenced specifically in any publications.
Publishing research using ab80589? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"