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Anti-GADD45G antibody (ab37480) at 1/2000 dilution + E coli derived fusion protein
Secondary
Goat anti IgY HRP at 1/1000 dilution
developed using the ECL technique
Predicted band size : 17 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
The arrow next to the image points to the GADD45G fusion protein.
The use of a fusion protien may account for the difference observed in molecular weight compared to the native protien.
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