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Products:Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Overview
Product name
Anti-GADD45G antibody
See all GADD45G products (4) ...
Description
Chicken polyclonal to GADD45G
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Full length protein (Human).
Positive control
E coli derived fusion protein.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgY
Research areas
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
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Western blot - GADD45G antibody (ab37480)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/2000. Detects a band of approximately 65 kDa (predicted molecular weight: 17 kDa).
We recommend using about 2.5 ug of positive control protein in the colorimetric method, and about 10x less for ECL detection.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK.
Sequence similarities
Belongs to the GADD45 family.
Target information above from: UniProt accessionO95257
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CR6 antibody
- Cytokine responsive protein CR6 antibody
- Cytokine-responsive protein CR6 antibody
see all
Database links
- Entrez Gene: 10912 Human
- Entrez Gene: 23882 Mouse
- Entrez Gene: 291005 Rat
- NCBI: NP_006696.1 Human
- Omim: 604949 Human
- SwissProt: O95257 Human
- SwissProt: Q5VZ87 Human
- SwissProt: Q5VZ88 Human
see all
Anti-GADD45G antibody images:
Western blot - GADD45G antibody (ab37480)
Anti-GADD45G antibody (ab37480) at 1/2000 dilution + E coli derived fusion protein
Secondary
Goat anti IgY HRP at 1/1000 dilution
developed using the ECL technique
Predicted band size : 17 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
The arrow next to the image points to the GADD45G fusion protein.
The use of a fusion protien may account for the difference observed in molecular weight compared to the native protien.
References for Anti-GADD45G antibody (ab37480)
ab37480 has not yet been referenced specifically in any publications.
Publishing research using ab37480? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"