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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Overview
Product name
Anti-GADD45GIP1 antibody
See all GADD45GIP1 products (4) ...
Description
Mouse polyclonal to GADD45GIP1
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length protein, corresponding to amino acids 1-222 of Human GADD45GIP1 (NP_443082)
Positive control
Transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Co-activators/co-repressors
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
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Western blot - GADD45GIP1 antibody (ab73111)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab73111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 25 kDa.
Target
Function
Acts as a negative regulator of G1 to S cell cycle phase progression by inhibiting cyclin-dependent kinases. Inhibitory effects are additive with GADD45 proteins but occurs also in the absence of GADD45 proteins. Acts as a repressor of the orphan nuclear receptor NR4A1 by inhibiting AB domain-mediated transcriptional activity. May be involved in the hormone-mediated regulation of NR4A1 transcriptional activity.
Tissue specificity
Widely expressed. Highly expressed in the thyroid gland, heart, lymph nodes, trachea and adrenal tissues. Expressed at lower level in liver skeletal muscle, kidney, pancreas, testis, ovary and stomach. Barely detectable in adrenal adenoma and papillary thyroid cancer.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ8TAE8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CKBBP2 antibody
- CKII beta associating protein antibody
- CKII beta binding protein 2 antibody
see all
Database links
- Entrez Gene: 90480 Human
- Omim: 605162 Human
- SwissProt: Q8TAE8 Human
- Unigene: 515164 Human
Anti-GADD45GIP1 antibody images:
Western blot - GADD45GIP1 antibody (ab73111)
All lanes : Anti-GADD45GIP1 antibody (ab73111) at 1/500 dilution
Lane 1 : GADD45GIP1-transfected 293T cell lysate (24.42 KDa)
Lane 2 : non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
HRP-conjugated goat anti-mouse at 1/2500 dilution
Predicted band size : 25 kDa
References for Anti-GADD45GIP1 antibody (ab73111)
ab73111 has not yet been referenced specifically in any publications.
Publishing research using ab73111? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"