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Western blot - GADD45GIP1 antibody (ab88769)
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Western blot - GADD45GIP1 antibody (ab88769)
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Product Name
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GADD45GIP1 antibody
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See all GADD45GIP1 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to GADD45GIP1
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Immunogen
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Full length protein, corresponding to amino acids 1-222 of Human GADD45GIP1 (NP_443082).
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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A431 lysate, transfected 293T cell lysate
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Co-activators/co-repressors
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
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Relevance
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Acts as a negative regulator of G1 to S cell cycle phase progression by inhibiting cyclin dependent kinases. Inhibitory effects are additive with GADD45 proteins but occurs also in the absence of GADD45 proteins. Acts as a repressor of the orphan nuclear receptor NR4A1 by inhibiting AB domain mediated transcriptional activity. May be involved in the hormone mediated regulation of NR4A1 transcriptional activity.
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GADD45GIP1 antibody (ab88769) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 1X PBS, pH 7.2
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GADD45GIP1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for GADD45GIP1 antibody images, references, products related to ab88769 and other tools.
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GADD45GIP1 antibody images:
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 Western blot - GADD45GIP1 antibody (ab88769)
GADD45GIP1 antibody (ab88769) at 1 µg + A431 cell lysate at 50 µg
Predicted band size : 25 kDa
Observed band size : 25 kDa
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Western blot - GADD45GIP1 antibody (ab88769)
All lanes : GADD45GIP1 antibody (ab88769) at 1 µg/ml
Lane 1 : transfected 293T lysate
Lane 2 : non transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Predicted band size : 25 kDa
Observed band size : 23 kDa (why is the actual band size different from the predicted?)
Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to GADD45GIP1
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GADD45GIP1 antibody - more information
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Customer reviews (feedback) regarding GADD45GIP1 antibody |
Customer FAQs regarding GADD45GIP1 antibody |
Protocols for GADD45GIP1 antibody |
Price and availability of products related to GADD45GIP1 antibody |
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GADD45GIP1 antibody - related products:
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GADD45GIP1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about GADD45GIP1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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