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Products:Cancer >> Tumor immunology >> Tumor-associated antigens
Overview
Product name
Anti-GAGE1 antibody
Description
Mouse polyclonal to GAGE1
Specificity
ab67412 is reactive against mammalian transfected lysate.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length protein, corresponding to amino acids 1-139 of Human GAGE1
Positive control
GAGE1 Transfected 293T lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
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Western blot - GAGE1 antibody (ab67412)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab67412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 27 kDa (predicted molecular weight: 15 kDa).
Target
Relevance
The GAGE1 gene belongs to a family of genes that are expressed in a variety of tumors but not in normal tissues, except for the testis. The sequences of the family members are highly related but differ by scattered nucleotide substitutions. The GAGE1 cDNA contains a 143-bp insertion, located in the coding sequence near the termination codon, that is absent from the other cDNAs. Autologous cytolytic T lymphocytes recognise the antigenic peptide YRPRPRRY, which is also encoded by several other family members. Nothing is presently known about the function of this protein. An alternatively spliced transcript variant has been found for this gene.
Alternative names
- Cancer/testis antigen 4.1 antibody
- CT4 1 antibody
- G antigen 1 antibody
see all
Database links
- Entrez Gene: 2543 Human
- Omim: 300594 Human
- SwissProt: Q13065 Human
- Unigene: 632815 Human
Anti-GAGE1 antibody images:
Western blot - GAGE1 antibody (ab67412)
All lanes : Anti-GAGE1 antibody (ab67412) at 1/500 dilution
Lane 1 : GAGE1 transfected 293T lysate
Lane 2 : Non transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 15 kDa
Observed band size : 27 kDa (why is the actual band size different from the predicted?)
References for Anti-GAGE1 antibody (ab67412)
ab67412 has not yet been referenced specifically in any publications.
Publishing research using ab67412? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"