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Western blot - GAGE1 antibody (ab67412)
(enlarge)
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Product Name
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GAGE1 antibody
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to GAGE1
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Immunogen
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Full length protein, corresponding to amino acids 1-139 of Human GAGE1
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Reacts with
(species key)
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Hu
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Specificity
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ab67412 is reactive against mammalian transfected lysate.
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 15 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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GAGE1 Transfected 293T lysate
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Research areas
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Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
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Relevance
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The GAGE1 gene belongs to a family of genes that are expressed in a variety of tumors but not in normal tissues, except for the testis. The sequences of the family members are highly related but differ by scattered nucleotide substitutions. The GAGE1 cDNA contains a 143-bp insertion, located in the coding sequence near the termination codon, that is absent from the other cDNAs. Autologous cytolytic T lymphocytes recognise the antigenic peptide YRPRPRRY, which is also encoded by several other family members. Nothing is presently known about the function of this protein. An alternatively spliced transcript variant has been found for this gene.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 1X PBS, pH 7.2
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this GAGE1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for GAGE1 antibody images, references, products related to ab67412 and other tools.
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GAGE1 antibody images:
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 Western blot - GAGE1 antibody (ab67412)
All lanes : GAGE1 antibody (ab67412) at 1/500 dilution
Lane 1 : GAGE1 transfected 293T lysate
Lane 2 : Non transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 15 kDa
Observed band size : 27 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to GAGE1
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GAGE1 antibody - more information
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Customer reviews (feedback) regarding GAGE1 antibody |
Customer FAQs regarding GAGE1 antibody |
Protocols for GAGE1 antibody |
Price and availability of products related to GAGE1 antibody |
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GAGE1 antibody - related products:
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GAGE1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about GAGE1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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