Anti-GAL4 antibody [15-6E10A7] - DNA Binding Domain (ab135397)

Overview

• Product nameAnti-GAL4 antibody [15-6E10A7] - DNA Binding DomainSee all GAL4 primary antibodies ...
• Description
  Mouse monoclonal [15-6E10A7] to GAL4 - DNA Binding Domain
• Tested applicationsWB, Other more details
• Species reactivity
  Reacts with: Saccharomyces cerevisiae
  
• Immunogen

  GAL4 DNA binding mini protein (1-147 amino acids) tagged to Maltose binding protein

  Run BLAST with Run BLAST with
• General notes

  
  

  The GAL4 DNA-BD monoclonal antibody binds specifically to the DNA-binding domain of the yeast GAL4 protein. This antibody is useful for verifying the expression and correct molecular weight of bait fusion proteins containing GAL4 DNA BD as the fusion partner in yeast two hybrid studies. The binding domain is typically used in a two-hybrid experiment as the fusion partner for the protein of interest. When the fusion protein of interest cannot activate transcription on its own, it can be used as bait to screen a library of cDNA clones that are fused to an activation domain. GAL4 is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes. Subunit structure: Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17. Domain: The 9aaTAD motif (residues 862 to 870) is a transactivation domain present in a large number of yeast and animal transcription factors. Post-translational modification: Association between GAL11 and GAL4 may serve to expedite phosphorylation of GAL4.

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.02% Sodium azide
• Concentration information loading...
• PurityAmmonium Sulphate Precipitation
• Primary antibody notes The GAL4 DNA-BD monoclonal antibody binds specifically to the DNA-binding domain of the yeast GAL4 protein. This antibody is useful for verifying the expression and correct molecular weight of bait fusion proteins containing GAL4 DNA BD as the fusion partner in yeast two hybrid studies. The binding domain is typically used in a two-hybrid experiment as the fusion partner for the protein of interest. When the fusion protein of interest cannot activate transcription on its own, it can be used as bait to screen a library of cDNA clones that are fused to an activation domain. GAL4 is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes. Subunit structure: Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17. Domain: The 9aaTAD motif (residues 862 to 870) is a transactivation domain present in a large number of yeast and animal transcription factors. Post-translational modification: Association between GAL11 and GAL4 may serve to expedite phosphorylation of GAL4.
• Clonality Monoclonal
• Clone number15-6E10A7
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Carbohydrate metabolism

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol II Transcription
  • Other

Applications

Target

• RelevanceFunction: This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes. Subunit structure: Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17. Domain: The 9aaTAD motif (residues 862 to 870) is a transactivation domain present in a large number of yeast and animal transcription factors. Post-translational modification: Association between GAL11 and GAL4 may serve to expedite phosphorylation of GAL4.
• Cellular localizationNuclear
• Database links
  • Entrez Gene: 855828 Saccharomyces cerevisiae
  • SwissProt: P04386 Saccharomyces cerevisiae

• Alternative names
  Gal4p antibodyGAL81 antibodyRegulatory protein GAL4 antibody

Anti-GAL4 antibody [15-6E10A7] - DNA Binding Domain images

• 

  Western blot - Anti-GAL4 antibody [15-6E10A7] - DNA Binding Domain (ab135397)
  All lanes : Anti-GAL4 antibody [15-6E10A7] - DNA Binding Domain (ab135397) at 1 µg/ml
  
  Lane 1 : Whole cell yeast lysate control from untransformed SEY6210 pep4-3 strain (a kind gift from Prof. Tom Stevens, University of Oregon).
  Lane 2 : Whole cell yeast lysate from SEY6210 pep4-3 strain transformed with pJK22-pGBDU plasmid containing GAL4 DNA Binding domain fused to the VPS60 Gene (a kind gift from Prof. Tom Stevens, University of Oregon).
  Lane 3 : Whole cell yeast lysate from SEY6210 pep4-3 strain transformed with pJk23-pGAD plasmid containing GAL4 Active Domain fused to the VPS60 Gene (a kind gift from Prof. Tom Stevens, University of Oregon).
  
  Secondary
  Anti-Mouse IgG (H&L) Goat Polyclonal
  
  Performed under reducing conditions.
  
  Predicted band size : 99 kDa
  Additional bands at : 56 kDa (possible isoform,Fusion protein).
  
  Exposure time : 3 minutes