Anti-GAPDH antibody [1A10A10] (ab75479)

Overview

• Product nameAnti-GAPDH antibody [1A10A10]See all GAPDH primary antibodies ...
• Description
  Mouse monoclonal [1A10A10] to GAPDH
• Tested applicationsIHC-Fr, WB, ELISA, IHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant full length protein (Human)

• Positive controlHuman hepatocarcinoma tissue; HepG2, A375, A2058, NCI-H460 and CEM cell line lysates

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: Ascites
• Concentration information loading...
• PurityAscites
• Clonality Monoclonal
• Clone number1A10A10
• Research Areas
  • Metabolism
  • Types of disease
  • Neurodegenerative disease

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Carbohydrate metabolism

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of carbohydrates

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Other

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Huntington's disease

Applications

Target

• FunctionHas both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
• PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
• Sequence similaritiesBelongs to the glyceraldehyde-3-phosphate dehydrogenase family.
• Post-translational
  modificationsS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
  ISGylated.
• Cellular localizationCytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.

Target information above from: UniProt accession P04406 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2597 Human
  • Omim: 138400 Human
  • SwissProt: P04406 Human
  • Unigene: 544577 Human
  • Unigene: 592355 Human
  • Unigene: 598320 Human

• Alternative names
  38 kDa BFA-dependent ADP-ribosylation substrate antibodyAging-associated gene 9 protein antibodyBARS-38 antibody

  cb609 antibodyEC 1.2.1.12 antibodyG3P_HUMAN antibodyG3PD antibodyG3PDH antibodyGAPD antibodyGAPDH antibodyGAPDH antibodyGlyceraldehyde 3 phosphate dehydrogenase antibodyGlyceraldehyde-3-phosphate dehydrogenase antibodyKNC-NDS6 antibodyMGC102544 antibodyMGC102546 antibodyMGC103190 antibodyMGC103191 antibodyMGC105239 antibodyMGC127711 antibodyMGC88685 antibodyOCAS, p38 component antibodyOCT1 coactivator in S phase, 38-KD component antibodyPeptidyl-cysteine S-nitrosylase GAPDH antibodywu:fb33a10 antibody

  see all

Anti-GAPDH antibody [1A10A10] images

• 

  Western blot - GAPDH antibody [1A10A10] (ab75479)
  All lanes : Anti-GAPDH antibody [1A10A10] (ab75479) at 1/25000 dilution
  
  Lane 1 : HepG2 cell line lysate
  Lane 2 : A375 cell line lysate
  Lane 3 : A2058 cell line lysate
  Lane 4 : NCI-H460 cell line lysate
  Lane 5 : CEM cell line lysate
  
  Lysates/proteins at 35 µg per lane.
  
  
  Predicted band size : 36 kDa
  Observed band size : 36 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GAPDH antibody [1A10A10] (ab75479)
  Immunohistochemistry analysis of two different samples of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue with 1/1000 ab75479 (a peroxidase-conjugated secondary antibody and DAB staining were used for detection).