Anti-GAPDH antibody [1A10A10] (ab75479)
- Product nameAnti-GAPDH antibody [1A10A10]See all GAPDH primary antibodies ...
- DescriptionMouse monoclonal [1A10A10] to GAPDH
- Tested applicationsIHC-Fr, WB, ELISA, IHC-P more details
- Species reactivityReacts with: Human
Recombinant full length protein (Human)
- Positive controlHuman hepatocarcinoma tissue; HepG2, A375, A2058, NCI-H460 and CEM cell line lysates
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
- Concentration information loading...
- Clonality Monoclonal
- Clone number1A10A10
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab75479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||IHC-Fr: Use at an assay dependent concentration. PubMed: 22125622|
|WB||WB: 1/5000 - 1/20000. Predicted molecular weight: 36 kDa.|
|ELISA||ELISA: Use at an assay dependent dilution.|
|IHC-P||IHC-P: 1/1000 - 1/10000.|
- FunctionHas both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
- PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
- Sequence similaritiesBelongs to the glyceraldehyde-3-phosphate dehydrogenase family.
modificationsS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
- Cellular localizationCytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
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Anti-GAPDH antibody [1A10A10] images
All lanes : Anti-GAPDH antibody [1A10A10] (ab75479) at 1/25000 dilution
Lane 1 : HepG2 cell line lysate
Lane 2 : A375 cell line lysate
Lane 3 : A2058 cell line lysate
Lane 4 : NCI-H460 cell line lysate
Lane 5 : CEM cell line lysate
Lysates/proteins at 35 µg per lane.
Predicted band size : 36 kDa
Observed band size : 36 kDa
Immunohistochemistry analysis of two different samples of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue with 1/1000 ab75479 (a peroxidase-conjugated secondary antibody and DAB staining were used for detection).
References for Anti-GAPDH antibody [1A10A10] (ab75479)
This product has been referenced in:
- O'Dwyer D et al. The proteomics of colorectal cancer: identification of a protein signature associated with prognosis. PLoS One 6:e27718 (2011). IHC-Fr ; Human . Read more (PubMed: 22125622) »
- Taylor LD et al. Biological characterization of Chlamydia trachomatis plasticity zone MACPF domain family protein CT153. Infect Immun 78:2691-9 (2010). WB ; Human . Read more (PubMed: 20351143) »