![Western blot - GAPDH antibody [3E8AD9 ] (ab110305) image](#GAPDH-Primary-antibodies-ab110305-1.gif)
![Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [3E8AD9 ] (ab110305) image](#GAPDH-Primary-antibodies-ab110305-2.gif)
![Immunoprecipitation - GAPDH antibody [3E8AD9 ] (ab110305) image](#GAPDH-Primary-antibodies-ab110305-3.gif)
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Product name
Anti-GAPDH antibody [3E8AD9 ]
See all GAPDH products (26) ...
Description
Mouse monoclonal [3E8AD9 ] to GAPDH
Tested applications
ICC/IF, WB, Flow Cyt, IP, In-Cell ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Purified protein
Positive control
WB: HepG2 cells IP: HepG2 cells and Human liver mitichondria Flow Cyt: HL-60 cells ICC/IF: Human HDFn cells
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
ab110305 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
3E8AD9
Isotype
IgG2b
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Types of disease >> Neurodegenerative disease
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Carbohydrate metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Energy Metabolism
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Huntington's disease
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 0.5 µg/ml.
WB: Use a concentration of 0.125 µg/ml. Predicted molecular weight: 36 kDa.
Flow Cyt: Use a concentration of 1 µg/ml.
IP: Use at an assay dependent concentration.
In-Cell ELISA: Use a concentration of 0.5 µg/ml. (0.05 µg/well).
Target
Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Sequence similarities
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
Post-translational
modifications
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated.
Cellular localization
Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
Target information above from: UniProt accessionP04406
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
- Aging-associated gene 9 protein antibody
- BARS-38 antibody
see all
Database links
- Entrez Gene: 2597 Human
- Omim: 138400 Human
- SwissProt: P04406 Human
- Unigene: 544577 Human
- Unigene: 592355 Human
- Unigene: 598320 Human
Anti-GAPDH antibody [3E8AD9 ] images:
Western blot - GAPDH antibody [3E8AD9 ] (ab110305)
![Western blot - GAPDH antibody [3E8AD9 ] (ab110305)](/ps/datasheet/images/110/ab110305/GAPDH-Primary-antibodies-ab110305-1.gif)
Anti-GAPDH antibody [3E8AD9 ] (ab110305) at 0.125 µg/ml + HepG2 cell lysate
Predicted band size : 36 kDa
Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [3E8AD9 ] (ab110305)
![Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody [3E8AD9 ] (ab110305)](/ps/datasheet/images/110/ab110305/GAPDH-Primary-antibodies-ab110305-2.gif)
Immunocytochemistry analysis using ab110305 at 0.5 µg/ml to stain GAPDH in HDFn cells (paraformaldehyde fixed and Triton X-100 permeabilized). Target locates primarily in the cytosol.
Immunoprecipitation - GAPDH antibody [3E8AD9 ] (ab110305)
![Immunoprecipitation - GAPDH antibody [3E8AD9 ] (ab110305)](/ps/datasheet/images/110/ab110305/GAPDH-Primary-antibodies-ab110305-3.gif)
Detection of ab110305 by Western Blot of Immunprecipitate.
Staining of GAPDH in HepG2 cells (lane 1) and Humman Liver Mitochondira (lane 2), immunoprecipitated using ab110305.
Flow Cytometry - GAPDH antibody [3E8AD9 ] (ab110305)
![Flow Cytometry - GAPDH antibody [3E8AD9 ] (ab110305)](/ps/datasheet/images/110/ab110305/GAPDH-Primary-antibodies-ab110305-4.gif)
Flow cytometric analysis of GAPDH in HL60 cells (blue), using ab110305 at 1 µg/ml. Isotype control antibody (red).
References for Anti-GAPDH antibody [3E8AD9 ] (ab110305)
This product has been referenced in:
- Whelan RS et al. Bax regulates primary necrosis through mitochondrial dynamics. Proc Natl Acad Sci U S A 109:6566-71 (2012).Read more (PubMed: 22493254) »
See 1 publication for this product
Publishing research using ab110305? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"