Overview
- Product nameAnti-GAPDH [6C5] antibodySee all GAPDH primary antibodies ...
- DescriptionMouse monoclonal [6C5] to GAPDH
- SpecificityThis antibody can be used as a loading control antibody, however we also recommend ab9484 as an alternative. GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. There is no cross-reaction with GAPDH from yeast. Preliminary data indicates that the antibody recognizes the monomer (36 kDa) and also the dimer forms of GAPDH, but not the tetrameric form of the protein.
- Tested applicationsELISA, ICC, WB, ICC/IF, IHC-Fr more details
- Species reactivityReacts with: Mouse, Rat, Rabbit, Chicken, Hamster, Dog, Human, Pig, Xenopus laevis, Fish, Monkey, Zebrafish, Baboon
Does not react with
Goat, Cow, Saccharomyces cerevisiae - Immunogen
Rabbit muscle GAPDH.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... - PurityProtein A purified
- Purification notesPurified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.
- Clonality Monoclonal
- Clone number6C5
- MyelomaSp2/0
- IsotypeIgG1
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab8245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA: Use at an assay dependent dilution. |
| ICC | ICC: Use at an assay dependent dilution. |
| WB | WB: 1/500 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 40.2 kDa). PubMed: 16450009 |
| ICC/IF | ICC/IF: Use at an assay dependent dilution. |
| IHC-Fr | IHC-Fr: 1/500. |
Target
- FunctionHas both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
- PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
- Sequence similaritiesBelongs to the glyceraldehyde-3-phosphate dehydrogenase family.
- Post-translational
modificationsS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated. - Cellular localizationCytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
-
Database links
- Entrez Gene: 374193 Chicken
- Entrez Gene: 403755 Dog
- Entrez Gene: 2597 Human
- Entrez Gene: 100042025 Mouse
- Entrez Gene: 14433 Mouse
- Entrez Gene: 396823 Pig
- Entrez Gene: 100009074 Rabbit
- Entrez Gene: 24383 Rat
- Entrez Gene: 685186 Rat
- Entrez Gene: 380259 Xenopus laevis
- Entrez Gene: 317743 Zebrafish
- Entrez Gene: 317743 Zebrafish
- Omim: 138400 Human
- SwissProt: P00356 Chicken
- SwissProt: Q28259 Dog
- SwissProt: P04406 Human
- SwissProt: P16858 Mouse
- SwissProt: P00355 Pig
- SwissProt: P46406 Rabbit
- SwissProt: P04797 Rat
- SwissProt: P51469 Xenopus laevis
- SwissProt: Q5XJ10 Zebrafish
- Unigene: 544577 Human
- Unigene: 592355 Human
- Unigene: 598320 Human
- Unigene: 304088 Mouse
- Unigene: 309092 Mouse
- Unigene: 317779 Mouse
- Unigene: 343110 Mouse
- Unigene: 392463 Mouse
- Unigene: 392480 Mouse
- Unigene: 414470 Mouse
- Unigene: 458138 Mouse
- Unigene: 458416 Mouse
- Unigene: 475698 Mouse
- Unigene: 129558 Rat
- Unigene: 91450 Rat
- Unigene: 995 Xenopus laevis
- Unigene: 35640 Zebrafish
see all
Target information above from: UniProt accession
P04406
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- 38 kDa BFA-dependent ADP-ribosylation substrate antibodyAging-associated gene 9 protein antibodyBARS-38 antibody
- cb609 antibodyEC 1.2.1.12 antibodyG3P_HUMAN antibodyG3PD antibodyG3PDH antibodyGAPD antibodyGAPDH antibodyGAPDH antibodyGlyceraldehyde 3 phosphate dehydrogenase antibodyGlyceraldehyde 3 phosphate dehydrogenase liver antibodyGlyceraldehyde 3 phosphate dehydrogenase muscle antibodyGlyceraldehyde-3-phosphate dehydrogenase antibodyKNC-NDS6 antibodyMGC102544 antibodyMGC102546 antibodyMGC103190 antibodyMGC103191 antibodyMGC105239 antibodyMGC127711 antibodyMGC88685 antibodyOCAS, p38 component antibodyOCT1 coactivator in S phase, 38-KD component antibodyPeptidyl-cysteine S-nitrosylase GAPDH antibodywu:fb33a10 antibody
see all
Anti-GAPDH [6C5] antibody images
-
![Western blot - GAPDH antibody [6C5] (ab8245)](http://a.abcam.com/ps/datasheet/Images/8/ab8245/ab8245_2.jpg)
Western blot - GAPDH antibody [6C5] (ab8245)All lanes : Anti-GAPDH [6C5] antibody (ab8245) at 2.5 µg/ml
Lane 1 : HeLa Nuclear
Lane 2 : HeLa whole cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HEK293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor anti-mouse at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 40.2 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?) -
![Western blot - GAPDH antibody [6C5] (ab8245)](http://a.abcam.com/ps/datasheet/Images/8/ab8245/ab8245_4.jpg)
Western blot - GAPDH antibody [6C5] (ab8245)All lanes : Anti-GAPDH [6C5] antibody (ab8245) at 1/20000 dilution
Lane 1 : Rabbit aorta, whole tissue lysate at 10 µg
Lane 2 : Blank
Lane 3 : Mouse aorta, whole tissue lysate at 5 µg
Lane 4 : Mouse aorta, whole tissue lysate at 7.5 µg
Lane 5 : Mouse aorta, whole tissue lysate at 10 µg
Lane 6 : Mouse aorta, whole tissue lysate at 12.5 µg
Lane 7 : Mouse aorta, whole tissue lysate at 15 µg
Secondary
HRP conjugated sheep anti-mouse antibody at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 40.2 kDa
Exposure time : 30 secondsThis image is courtesy of an anonymous Abreview
-
![Western blot - GAPDH antibody [6C5] (ab8245)](http://a.abcam.com/ps/datasheet/Images/8/ab8245/ab8245_1.gif)
Western blot - GAPDH antibody [6C5] (ab8245)
Predicted band size : 40.2 kDa
20 µg of Raji whole cell lysate (ab7908) probed with mouse monoclonal to GAPDH (ab8245) at 10µg/ml.
References for Anti-GAPDH [6C5] antibody (ab8245)
This product has been referenced in:
- Du M et al. SB225002 promotes mitotic catastrophe in chemo-sensitive and -resistant ovarian cancer cells independent of p53 status in vitro. PLoS One 8:e54572 (2013). WB ; Human . Read more (PubMed: 23359652) »
- Schulz R et al. Inhibiting the HSP90 chaperone destabilizes macrophage migration inhibitory factor and thereby inhibits breast tumor progression. J Exp Med 209:275-89 (2012). Read more (PubMed: 22271573) »
