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Anti-GAPDH antibody [6C5] (ab8245)

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Overview

Product name

Anti-GAPDH antibody [6C5]
See all GAPDH products (26) ...

Description

Mouse monoclonal [6C5] to GAPDH

Specificity

This antibody can be used as a loading control antibody, however we also recommend ab9484 as an alternative. GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. There is no cross-reaction with GAPDH from yeast. Preliminary data indicates that the antibody recognizes the monomer (36 kDa) and also the dimer forms of GAPDH, but not the tetrameric form of the protein.

Tested applications

ELISA, ICC, WB, ICC/IF, IHC-Frmore details

Cross reactivity

Reacts with

Mouse, Rat, Rabbit, Chicken, Dog, Human, Pig, Xenopus laevis, Fish, Monkey, Zebrafish, Baboon

Does not react with

Goat, Cow, Saccharomyces cerevisiae

Immunogen

Rabbit muscle GAPDH.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein A purified

Purification notes

Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.

Clonality

Monoclonal

Clone number

6C5

Myeloma

Sp2/0

Isotype

IgG1

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Types of disease >> Neurodegenerative disease
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Carbohydrate metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Energy Metabolism
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Huntington's disease

  • Western blot - GAPDH antibody [6C5] (ab8245)Western blot - GAPDH antibody [6C5] (ab8245) image (enlarge)

  • Western blot - GAPDH antibody [6C5] (ab8245)Western blot - GAPDH antibody [6C5] (ab8245) image (enlarge)

  • Western blot - GAPDH antibody [6C5] (ab8245)Western blot - GAPDH antibody [6C5] (ab8245) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab8245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.

Sequence similarities

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Post-translational
modifications

S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated.

Cellular localization

Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.

Target information above from: UniProt accessionP04406 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 38 kDa BFA-dependent ADP-ribosylation substrate antibody
  • Aging-associated gene 9 protein antibody
  • BARS-38 antibody
  • cb609 antibody
  • EC 1.2.1.12 antibody
  • G3P_HUMAN antibody
  • G3PD antibody
  • G3PDH antibody
  • GAPD antibody
  • GAPDH antibody
  • GAPDH antibody
  • Glyceraldehyde 3 phosphate dehydrogenase antibody
  • Glyceraldehyde 3 phosphate dehydrogenase liver antibody
  • Glyceraldehyde 3 phosphate dehydrogenase muscle antibody
  • Glyceraldehyde-3-phosphate dehydrogenase antibody
  • KNC-NDS6 antibody
  • MGC102544 antibody
  • MGC102546 antibody
  • MGC103190 antibody
  • MGC103191 antibody
  • MGC105239 antibody
  • MGC127711 antibody
  • MGC88685 antibody
  • OCAS, p38 component antibody
  • OCT1 coactivator in S phase, 38-KD component antibody
  • Peptidyl-cysteine S-nitrosylase GAPDH antibody
  • wu:fb33a10 antibody
see all

Database links

see all

Anti-GAPDH antibody [6C5] images:

  Western blot - GAPDH antibody [6C5] (ab8245)

Western blot - GAPDH antibody [6C5] (ab8245)

All lanes : Anti-GAPDH antibody [6C5] (ab8245) at 2.5 µg/ml

Lane 1 : HeLa Nuclear
Lane 2 : HeLa whole cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HEK293 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Alexa Fluor anti-mouse at 1/5000 dilution

Performed under reducing conditions.

Predicted band size : 40.2 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)


Fluorescence detection of secondary antibody.

  Western blot - GAPDH antibody [6C5] (ab8245)

Western blot - GAPDH antibody [6C5] (ab8245)

All lanes : Anti-GAPDH antibody [6C5] (ab8245) at 1/20000 dilution

Lane 1 : Rabbit aorta, whole tissue lysate at 10 µg
Lane 2 : Blank
Lane 3 : Mouse aorta, whole tissue lysate at 5 µg
Lane 4 : Mouse aorta, whole tissue lysate at 7.5 µg
Lane 5 : Mouse aorta, whole tissue lysate at 10 µg
Lane 6 : Mouse aorta, whole tissue lysate at 12.5 µg
Lane 7 : Mouse aorta, whole tissue lysate at 15 µg

Secondary
HRP conjugated sheep anti-mouse antibody at 1/10000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 40.2 kDa


Exposure time : 30 seconds

This image is courtesy of an anonymous Abreview

See Abreview

  Western blot - GAPDH antibody [6C5] (ab8245)

Western blot - GAPDH antibody [6C5] (ab8245)



Predicted band size : 40.2 kDa


20 µg of Raji whole cell lysate (ab7908) probed with mouse monoclonal to GAPDH (ab8245) at 10µg/ml.

References for Anti-GAPDH antibody [6C5] (ab8245)

This product has been referenced in:

  • Schulz R  et al. Inhibiting the HSP90 chaperone destabilizes macrophage migration inhibitory factor and thereby inhibits breast tumor progression. J Exp Med 209:275-89 (2012).Read more (PubMed: 22271573) »
  • Luo J  et al. Role of FYN kinase in spermatogenesis: defects characteristic of Fyn-null sperm in mice. Biol Reprod 86:1-8 (2012).Read more (PubMed: 21918125) »

See all 124 publications for this product

Publishing research using ab8245? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"