Overview

  • Product nameAnti-GAPDH antibody - C-terminal
    See all GAPDH primary antibodies
  • Description
    Rabbit polyclonal to GAPDH - C-terminal
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide derived from the C terminal region of Human GAPDH.

  • Positive control
    • IM-9, ECL, SGC or Jurkat cell lysate.

Applications

Our Abpromise guarantee covers the use of ab93378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/3000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionHas both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
    • PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
    • Sequence similaritiesBelongs to the glyceraldehyde-3-phosphate dehydrogenase family.
    • Post-translational
      modifications
      S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
      ISGylated.
    • Cellular localizationCytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
    • Information by UniProt
    • Database links
    • Alternative names
      • 38 kDa BFA-dependent ADP-ribosylation substrate antibody
      • aging associated gene 9 protein antibody
      • Aging-associated gene 9 protein antibody
      • BARS-38 antibody
      • cb609 antibody
      • EC 1.2.1.12 antibody
      • Epididymis secretory sperm binding protein Li 162eP antibody
      • G3P_HUMAN antibody
      • G3PD antibody
      • G3PDH antibody
      • GAPD antibody
      • GAPDH antibody
      • GAPDH antibody
      • Glyceraldehyde 3 phosphate dehydrogenase antibody
      • Glyceraldehyde-3-phosphate dehydrogenase antibody
      • HEL-S-162eP antibody
      • KNC-NDS6 antibody
      • MGC102544 antibody
      • MGC102546 antibody
      • MGC103190 antibody
      • MGC103191 antibody
      • MGC105239 antibody
      • MGC127711 antibody
      • MGC88685 antibody
      • OCAS, p38 component antibody
      • OCT1 coactivator in S phase, 38-KD component antibody
      • OTTHUMP00000174431 antibody
      • OTTHUMP00000174432 antibody
      • peptidyl cysteine S nitrosylase GAPDH antibody
      • Peptidyl-cysteine S-nitrosylase GAPDH antibody
      • wu:fb33a10 antibody
      see all

    Anti-GAPDH antibody - C-terminal images

    • All lanes : Anti-GAPDH antibody - C-terminal (ab93378) at 1/1000 dilution

      Lane 1 : IM-9 cell lysate
      Lane 2 : SGC cell lysate
      Lane 3 : ECL cell lysate

      Lysates/proteins at 50 µg per lane.


      Predicted band size : 36 kDa
      Observed band size : 36 kDa

    References for Anti-GAPDH antibody - C-terminal (ab93378)

    This product has been referenced in:
    • Tsvetanova NG  et al. The yeast Rab GTPase Ypt1 modulates unfolded protein response dynamics by regulating the stability of HAC1 RNA. PLoS Genet 8:e1002862 (2012). Read more (PubMed: 22844259) »

    See 1 Publication for this product

    Product Wall

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    I am sorry to see that you have been having problems with multiple banding using ab93378. The lower banding you have shown me in these images matched what we would expect from GAPDH. Were these blots reprobed. ...

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