The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 20 µg/ml.
Use a concentration of 10 µg/ml.
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 36 kDa).
FunctionHas both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Sequence similaritiesBelongs to the glyceraldehyde-3-phosphate dehydrogenase family.
Post-translational modificationsS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus. ISGylated.
Cellular localizationCytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
Western blot - GAPDH antibody - Carboxyterminal end (ab62489)
Lane 1 : Anti-GAPDH antibody - Carboxyterminal end (ab62489) at 0.5 µg/ml Lane 2 : Anti-GAPDH antibody - Carboxyterminal end (ab62489) at 1 µg/ml Lane 3 : Anti-GAPDH antibody - Carboxyterminal end (ab62489) at 2 µg/ml
Lane 1 : Hela cell lysate Lane 2 : Hela cell lysate Lane 3 : Hela cell lysate