Anti-GAPDH antibody - Carboxyterminal end (ab62489)

Overview

  • Product nameAnti-GAPDH antibody - Carboxyterminal endSee all GAPDH primary antibodies ...
  • Description
    Rabbit polyclonal to GAPDH - Carboxyterminal end
  • SpecificityThis antibody reacts with GAPDH
  • Tested applicationsICC/IF, ICC, WB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 16 amino acid synthetic peptide from near the carboxy-terminus of human GAPDH

  • Positive control
    • Hela cell lysates

Applications

Our Abpromise guarantee covers the use of ab62489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF Use a concentration of 20 µg/ml.
ICC Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 36 kDa).

Target

  • FunctionHas both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
  • PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
  • Sequence similaritiesBelongs to the glyceraldehyde-3-phosphate dehydrogenase family.
  • Post-translational
    modifications
    S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
    ISGylated.
  • Cellular localizationCytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
  • Target information above from: UniProt accession P04406 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Aging associated gene 9 protein antibody
    • G3P_HUMAN antibody
    • G3PD antibody
    • G3PDH antibody
    • GAPD antibody
    • GAPDH antibody
    • GAPDH antibody
    • Glyceraldehyde 3 phosphate dehydrogenase antibody
    • Glyceraldehyde 3 phosphate dehydrogenase liver antibody
    • Glyceraldehyde 3 phosphate dehydrogenase muscle antibody
    • Glyceraldehyde-3-phosphate dehydrogenase antibody
    • MGC88685 antibody
    • OCAS p38 component antibody
    • OCT1 coactivator in S phase 38 KD Component antibody
    • Peptidyl-cysteine S-nitrosylase GAPDH antibody
    see all

Anti-GAPDH antibody - Carboxyterminal end images

  • Lane 1 : Anti-GAPDH antibody - Carboxyterminal end (ab62489) at 0.5 µg/ml
    Lane 2 : Anti-GAPDH antibody - Carboxyterminal end (ab62489) at 1 µg/ml
    Lane 3 : Anti-GAPDH antibody - Carboxyterminal end (ab62489) at 2 µg/ml

    Lane 1 : Hela cell lysate
    Lane 2 : Hela cell lysate
    Lane 3 : Hela cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 36 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
  • ab62489 at 10µg/ml staining GAPDH in human Hela cells by Imunocytochemistry. 

  • Immunofluorescence of GAPDH in HeLa cells using ab62489 at 20 ug/ml.

References for Anti-GAPDH antibody - Carboxyterminal end (ab62489)

ab62489 has not yet been referenced specifically in any publications.

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