Anti-GAPDH antibody - Carboxyterminal end (ab62489)

Overview

• Product nameAnti-GAPDH antibody - Carboxyterminal endSee all GAPDH primary antibodies ...
• Description
  Rabbit polyclonal to GAPDH - Carboxyterminal end
• SpecificityThis antibody reacts with GAPDH
• Tested applicationsICC/IF, ICC, WB more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  A 16 amino acid synthetic peptide from near the carboxy-terminus of human GAPDH

• Positive controlHela cell lysates

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Neurodegenerative disease

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Carbohydrate metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of carbohydrates

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Isotype/Loading Controls
  • Loading Controls
  • GAPDH

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Other

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Huntington's disease

Applications

Target

• FunctionHas both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
• PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
• Sequence similaritiesBelongs to the glyceraldehyde-3-phosphate dehydrogenase family.
• Post-translational
  modificationsS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
  ISGylated.
• Cellular localizationCytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.

Target information above from: UniProt accession P04406 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2597 Human
  • Entrez Gene: 100042025 Mouse
  • Entrez Gene: 14433 Mouse
  • Entrez Gene: 24383 Rat
  • Entrez Gene: 685186 Rat
  • Omim: 138400 Human
  • SwissProt: P04406 Human
  • SwissProt: P16858 Mouse

  • SwissProt: P04797 Rat
  • Unigene: 544577 Human
  • Unigene: 592355 Human
  • Unigene: 598320 Human
  • Unigene: 304088 Mouse
  • Unigene: 309092 Mouse
  • Unigene: 317779 Mouse
  • Unigene: 343110 Mouse
  • Unigene: 392463 Mouse
  • Unigene: 392480 Mouse
  • Unigene: 414470 Mouse
  • Unigene: 458138 Mouse
  • Unigene: 458416 Mouse
  • Unigene: 475698 Mouse
  • Unigene: 129558 Rat
  • Unigene: 91450 Rat

  see all

• Alternative names
  Aging associated gene 9 protein antibodyG3P_HUMAN antibodyG3PD antibody

  G3PDH antibodyGAPD antibodyGAPDH antibodyGAPDH antibodyGlyceraldehyde 3 phosphate dehydrogenase antibodyGlyceraldehyde 3 phosphate dehydrogenase liver antibodyGlyceraldehyde 3 phosphate dehydrogenase muscle antibodyGlyceraldehyde-3-phosphate dehydrogenase antibodyMGC88685 antibodyOCAS p38 component antibodyOCT1 coactivator in S phase 38 KD Component antibodyPeptidyl-cysteine S-nitrosylase GAPDH antibody

  see all

Anti-GAPDH antibody - Carboxyterminal end images

• 

  Western blot - GAPDH antibody - Carboxyterminal end (ab62489)
  Lane 1 : Anti-GAPDH antibody - Carboxyterminal end (ab62489) at 0.5 µg/ml
  Lane 2 : Anti-GAPDH antibody - Carboxyterminal end (ab62489) at 1 µg/ml
  Lane 3 : Anti-GAPDH antibody - Carboxyterminal end (ab62489) at 2 µg/ml
  
  Lane 1 : Hela cell lysate
  Lane 2 : Hela cell lysate
  Lane 3 : Hela cell lysate
  
  Lysates/proteins at 15 µg per lane.
  
  
  Predicted band size : 36 kDa
  Observed band size : 39 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry - GAPDH antibody - Carboxyterminal end (ab62489)

  ab62489 at 10µg/ml staining GAPDH in human Hela cells by Imunocytochemistry. 

• 

  Immunocytochemistry/ Immunofluorescence-Anti-GAPDH antibody - Carboxyterminal end(ab62489)
  Immunofluorescence of GAPDH in HeLa cells using ab62489 at 20 ug/ml.