Anti-GAPDH antibody - Carboxyterminal end (ab77514)

Overview

  • Product nameAnti-GAPDH antibody - Carboxyterminal endSee all GAPDH primary antibodies ...
  • Description
    Rabbit polyclonal to GAPDH - Carboxyterminal end
  • Tested applicationsWB, IHC-P, IHC-Fr more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide from C terminal of rat GAPDH, conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in mouse and shares 90% identity with human sequence.

  • Positive control
    • Human neutrophil lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab77514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IHC-P
IHC-Fr
  • Application notesIHC-P: Use at a concentration of 10 - 50 µg/ml. For paraffin sections antigen retrieval is recommended.
    IHC-Fr: Use at a concentration of 10 - 50 µg/ml.
    WB: Use at a concentration of 10 - 50 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionHas both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
    • PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
    • Sequence similaritiesBelongs to the glyceraldehyde-3-phosphate dehydrogenase family.
    • Post-translational
      modifications
      S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
      ISGylated.
    • Cellular localizationCytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
    • Target information above from: UniProt accession P04406 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • 38 kDa BFA-dependent ADP-ribosylation substrate antibody
      • aging associated gene 9 protein antibody
      • Aging-associated gene 9 protein antibody
      • BARS-38 antibody
      • cb609 antibody
      • EC 1.2.1.12 antibody
      • G3P_HUMAN antibody
      • G3PD antibody
      • G3PDH antibody
      • GAPD antibody
      • GAPDH antibody
      • GAPDH antibody
      • Glyceraldehyde 3 phosphate dehydrogenase antibody
      • Glyceraldehyde-3-phosphate dehydrogenase antibody
      • KNC-NDS6 antibody
      • MGC102544 antibody
      • MGC102546 antibody
      • MGC103190 antibody
      • MGC103191 antibody
      • MGC105239 antibody
      • MGC127711 antibody
      • MGC88685 antibody
      • OCAS, p38 component antibody
      • OCT1 coactivator in S phase, 38-KD component antibody
      • peptidyl cysteine S nitrosylase GAPDH antibody
      • Peptidyl-cysteine S-nitrosylase GAPDH antibody
      • wu:fb33a10 antibody
      see all

    Anti-GAPDH antibody - Carboxyterminal end images

    • Anti-GAPDH antibody - Carboxyterminal end (ab77514) at 30 µg/ml + human neutrophil lysate

      Predicted band size : 36 kDa
      Observed band size : 36 kDa

    References for Anti-GAPDH antibody - Carboxyterminal end (ab77514)

    ab77514 has not yet been referenced specifically in any publications.

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