Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Huntington's disease
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ab9385 has been referenced in 9 publications.
Publishing research using ab9385? Please let us know so that we can cite the reference in this datasheet
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Lanes 1 - 4 : Anti-GAPDH antibody (HRP) - Loading Control (ab9385) at 1/5000 dilution ((Blocked in 5% BSA))
Lanes 5 - 8 : Anti-GAPDH antibody (HRP) - Loading Control (ab9385) at 1/5000 dilution ((Blocked in 5% MILK))
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab52254)
Lane 3 :
Lane 4 :
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)
Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab52254)
Lane 7 :
Lane 8 :
Lysates/proteins at 10 µg per lane.
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 36 kDa
The membrane 1-4 was blocked in 5% BSA (1 hour). The membrane 5-8 was blocked in 5% MILK (1 hour). Abcam routinely uses 5% BSA to block, however following recent customer feedback our labs investigated the effect of 5% milk blocking. We can now confirm that milk is not a suitable blocking agent for this antibody and significantly decreases the signal on the membrane.
All lanes : Anti-GAPDH antibody (HRP) - Loading Control (ab9385) at 1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size : 36 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
8
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