Anti-GAPDH antibody (HRP) - Loading Control (ab9385)
Overview
- Product nameAnti-GAPDH antibody (HRP) - Loading ControlSee all GAPDH primary antibodies ...
- DescriptionRabbit polyclonal to GAPDH - Loading Control (HRP)
- ConjugationHRP
- Conjugation notesThis antibody has been conjugated with HRP. The concentration of the original antibody was 1 mg/ml, following conjugation the concentration observed will be higher.
- Tested applicationsWB more details
- Species reactivityReacts with: Mouse, Rat, Human, Saccharomyces cerevisiae, Xenopus laevis
- Immunogen
Full length native protein from human erythrocytes.
- Positive controlThis antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; A431; Hek293.
- General notes
This antibody is the HRP conjugated version of ab9485, for more convenient use as a loading control antibody.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 50% Glycerol, PBS, pH 7.4
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Concentration information loading... - PurityImmunogen affinity purified
- Primary antibody notes This antibody is the HRP conjugated version of ab9485, for more convenient use as a loading control antibody.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab9385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa). Milk blocking may cause 'no bands' problem. Please try BSA as well. |
Target
- FunctionHas both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
- PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
- Sequence similaritiesBelongs to the glyceraldehyde-3-phosphate dehydrogenase family.
- Post-translational
modificationsS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated. - Cellular localizationCytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
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Database links
- Entrez Gene: 2597 Human
- Entrez Gene: 100042025 Mouse
- Entrez Gene: 14433 Mouse
- Entrez Gene: 24383 Rat
- Entrez Gene: 685186 Rat
- Entrez Gene: 380259 Xenopus laevis
- Omim: 138400 Human
- SwissProt: P04406 Human
- SwissProt: P16858 Mouse
- SwissProt: P04797 Rat
- SwissProt: P51469 Xenopus laevis
- Unigene: 544577 Human
- Unigene: 592355 Human
- Unigene: 598320 Human
- Unigene: 304088 Mouse
- Unigene: 309092 Mouse
- Unigene: 317779 Mouse
- Unigene: 343110 Mouse
- Unigene: 392463 Mouse
- Unigene: 392480 Mouse
- Unigene: 414470 Mouse
- Unigene: 458138 Mouse
- Unigene: 458416 Mouse
- Unigene: 475698 Mouse
- Unigene: 129558 Rat
- Unigene: 91450 Rat
- Unigene: 995 Xenopus laevis
see all
Target information above from: UniProt accession
P04406
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- Aging associated gene 9 protein antibodyG3P_HUMAN antibodyG3PD antibody
- G3PD antibodyG3PDH antibodyGAPD antibodyGAPDH antibodyGAPDH antibodyGlyceraldehyde 3 phosphate dehydrogenase antibodyGlyceraldehyde 3 phosphate dehydrogenase liver antibodyGlyceraldehyde 3 phosphate dehydrogenase muscle antibodyGlyceraldehyde-3-phosphate dehydrogenase antibodyMGC88685 antibodyOCAS p38 component antibodyOCT1 coactivator in S phase 38 KD Component antibodyPeptidyl-cysteine S-nitrosylase GAPDH antibody
see all
Anti-GAPDH antibody (HRP) - Loading Control images
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Western blot - GAPDH antibody (HRP) - Loading Control (ab9385)Lanes 1 - 4 : Anti-GAPDH antibody (HRP) - Loading Control (ab9385) at 1/5000 dilution ((Blocked in 5% BSA))
Lanes 5 - 8 : Anti-GAPDH antibody (HRP) - Loading Control (ab9385) at 1/5000 dilution ((Blocked in 5% MILK))
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab52254)
Lane 3 :A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
Lane 4 :HEK293 Human embryonic kidney cell line Whole Cell Lysate (ab7902)
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)
Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab52254)
Lane 7 :A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
Lane 8 :HEK293 Human embryonic kidney cell line Whole Cell Lysate (ab7902)
Lysates/proteins at 10 µg per lane.
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 36 kDa -
Western blot - GAPDH antibody (ab9385)All lanes : Anti-GAPDH antibody (HRP) - Loading Control (ab9385) at 1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size : 36 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
References for Anti-GAPDH antibody (HRP) - Loading Control (ab9385)
This product has been referenced in:
- Gamberi T et al. Evaluation of SCO1 deletion on Saccharomyces cerevisiae metabolism through a proteomic approach. Proteomics 12:1767-80 (2012). Read more (PubMed: 22623105) »
- Kim JY et al. Low molecular weight silk fibroin increases alkaline phosphatase and type I collagen expression in MG63 cells. BMB Rep 43:52-6 (2010). WB . Read more (PubMed: 20132736) »
