Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Huntington's disease
Overview
Product name
Anti-GAPDH antibody (HRP) - Loading Control
See all GAPDH products (9) ...
Description
Rabbit polyclonal to GAPDH - Loading Control (HRP)
Conjugation
HRP
Conjugation notes
This antibody has been conjugated with HRP. The concentration of the original antibody was 1 mg/ml, following conjugation the concentration observed will be higher.
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human, Saccharomyces cerevisiae, Xenopus laevis
Immunogen
Full length native protein from human erythrocytes.
Positive control
This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; A431; Hek293.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
This antibody is the HRP conjugated version of ab9485, for more convenient use as a loading control antibody.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Energy Metabolism
Isotype/Loading Controls >> Loading Controls >> GAPDH
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Huntington's disease
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/5000Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa).(Milk blocking may cause 'no bands' problem. Please try BSA as well.)
Target
Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Sequence similarities
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
Post-translational
modifications
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated.
Cellular localization
Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
Target information above from: UniProt accessionP04406
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Aging associated gene 9 protein antibody
- G3P_HUMAN antibody
- G3PD antibody
see all
Database links
- Entrez Gene: 2597 Human
- Entrez Gene: 100042025 Mouse
- Entrez Gene: 14433 Mouse
- Entrez Gene: 24383 Rat
- Entrez Gene: 685186 Rat
- Entrez Gene: 380259 Xenopus laevis
- Omim: 138400 Human
- SwissProt: P04406 Human
see all
Anti-GAPDH antibody (HRP) - Loading Control images:
Western blot - GAPDH antibody (HRP) - Loading Control (ab9385)
Lanes 1 - 4 : Anti-GAPDH antibody (HRP) - Loading Control (ab9385) at 1/5000 dilution ((Blocked in 5% BSA))
Lanes 5 - 8 : Anti-GAPDH antibody (HRP) - Loading Control (ab9385) at 1/5000 dilution ((Blocked in 5% MILK))
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab52254)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate (ab7902)
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)
Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab52254)
Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
Lane 8 : HEK293 Human embryonic kidney cell line Whole Cell Lysate (ab7902)
Lysates/proteins at 10 µg per lane.
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 36 kDa
The membrane 1-4 was blocked in 5% BSA (1 hour). The membrane 5-8 was blocked in 5% MILK (1 hour). Abcam routinely uses 5% BSA to block, however following recent customer feedback our labs investigated the effect of 5% milk blocking. We can now confirm that milk is not a suitable blocking agent for this antibody and significantly decreases the signal on the membrane.
Western blot - GAPDH antibody (ab9385)
All lanes : Anti-GAPDH antibody (HRP) - Loading Control (ab9385) at 1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size : 36 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
References for Anti-GAPDH antibody (HRP) - Loading Control (ab9385)
This product has been referenced in:
- Kim JYet al. Low molecular weight silk fibroin increases alkaline phosphatase and type I collagen expression in MG63 cells. BMB Rep 43:52-6 (2010). WB.Read more (PubMed: 20132736) »
- Heidrich FMet al. Chromogranin B regulates calcium signaling, nuclear factor kappaB activity, and brain natriuretic peptide production in cardiomyocytes. Circ Res 102:1230-8 (2008). WB; Mouse.Read more (PubMed: 18420944) »
See all 9 publications for this product
Publishing research using ab9385? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"