GAPDH antibody (HRP) - Loading Control (ab9385)

Product Name 

GAPDH antibody (HRP) - Loading Control

Product type 

Primary antibodies

Description 

Rabbit polyclonal to GAPDH - Loading Control (HRP)

Immunogen 

Full length native protein from human erythrocytes.

Reacts with 
(species key)

Hu, Ms, Rat, Sc, Xl

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
WB: 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Energy Metabolism
Isotype/Loading Controls >> Loading Controls >> GAPDH
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Huntington's disease

Relevance 

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age-related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed in almost all tissues at high levels, therefore becoming the marker of choice when a loading control in Western blotting is required. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

This antibody is the HRP conjugated version of ab9485, for more convenient use as a loading control antibody.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Conjugation notes 

This antibody has been conjugated with HRP. The concentration of the original antibody was 1 mg/ml, following conjugation the concentration observed will be higher.

Storage buffer 

Preservative: None
Constituents: 50% Glycerol, PBS

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Form 

Liquid

Conjugation 

Horse Radish Peroxidase

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

See below for GAPDH antibody (HRP) - Loading Control images, references, products related to ab9385 and other tools.

GAPDH antibody (HRP) - Loading Control images:

References for GAPDH antibody (HRP) - Loading Control (ab9385)

  This product has been used in: (two most recent references)

Kim JY et al. Low molecular weight silk fibroin increases alkaline phosphatase and type I collagen expression in MG63 cells. BMB Rep 43:52-6 (2010). WB. PubMed: 20132736

Heidrich FM et al. Chromogranin B regulates calcium signaling, nuclear factor kappaB activity, and brain natriuretic peptide production in cardiomyocytes. Circ Res 102:1230-8 (2008). WB; Mouse. PubMed: 18420944

See all 10 publication references for this product.


If you publish research using ab9385 please let us know so that we can cite the reference on this datasheet.

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