Overview

  • Product name
  • Description
    Goat polyclonal to GAPDH
  • Tested applications
    Suitable for: IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Turkey, Pig, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 175-225 of Human GAPDH (NP_002037.2).

  • Positive control
    • 293T, HeLa, Jurkat and mouse NIH 3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab157156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/2500. Predicted molecular weight: 36 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    Anti-GAPDH antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling GAPDH with ab157156 at 1/1000 (1µg/ml). Detection: DAB.
    • All lanes : Anti-GAPDH antibody (ab157156) at 1 µg/ml

      Lane 1 : 293T whole cell lysate at 50 µg
      Lane 2 : 293T whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 50 µg
      Lane 4 : Jurkat whole cell lysate at 50 µg
      Lane 5 : NIH 3T3 whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 36 kDa


      Exposure time : 30 seconds

    References for Anti-GAPDH antibody (ab157156)

    ab157156 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab157156.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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