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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab15822? Please let us know so that we can cite the reference in this datasheet
ab15822 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - GAPDH antibody (ab15822)
Anti-GAPDH antibody (ab15822) at 1 µg/ml + HeLa whole cell lysate at 20 µg
Secondary
Goat Anti-Chicken IgG (H&L) secondary at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 40 kDa
ab15822 detects a band of ~ 40 kDa in HeLa, Jurkat and A431 whole cell lysates.
Immunocytochemistry/ Immunofluorescence - GAPDH antibody (ab15822)
ICC/IF image of ab15822 stained human MCF7 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab15822, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-chicken IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and HepG2 cells.
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