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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - GAPDH antibody (ab15822)
Anti-GAPDH antibody (ab15822) at 1 µg/ml + HeLa whole cell lysate at 20 µg
Secondary
Goat Anti-Chicken IgG (H&L) secondary at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 40 kDa
ab15822 detects a band of ~ 40 kDa in HeLa, Jurkat and A431 whole cell lysates.
Immunocytochemistry/ Immunofluorescence - GAPDH antibody (ab15822)
ICC/IF image of ab15822 stained human MCF7 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab15822, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-chicken IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and HepG2 cells.
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