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Anti-GAPDH antibody (ab15822)

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Overview

Product name

Anti-GAPDH antibody
See all GAPDH products (9) ...

Description

Chicken polyclonal to GAPDH

Tested applications

WB, ICC/IFmore details

Cross reactivity

Reacts with

Human

Immunogen

GAPDH from human erythrocytes.

Positive control

This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; A431.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

IgY fraction

Clonality

Polyclonal

Isotype

IgY

  • Western blot - GAPDH antibody (ab15822)Western blot - GAPDH antibody (ab15822) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - GAPDH antibody (ab15822)Immunocytochemistry/ Immunofluorescence - GAPDH antibody (ab15822) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab15822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.

Sequence similarities

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Post-translational
modifications

S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated.

Cellular localization

Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.

Target information above from: UniProt accessionP04406 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Aging associated gene 9 protein antibody
  • G3P_HUMAN antibody
  • G3PD antibody
  • G3PD antibody
  • G3PDH antibody
  • G3PDH antibody
  • GAPD antibody
  • GAPD antibody
  • GAPDH antibody
  • GAPDH antibody
  • Glyceraldehyde 3 phosphate dehydrogenase antibody
  • Glyceraldehyde 3 phosphate dehydrogenase antibody
  • Glyceraldehyde 3 phosphate dehydrogenase liver antibody
  • Glyceraldehyde 3 phosphate dehydrogenase liver antibody
  • Glyceraldehyde 3 phosphate dehydrogenase muscle antibody
  • Glyceraldehyde 3 phosphate dehydrogenase muscle antibody
  • Glyceraldehyde-3-phosphate dehydrogenase antibody
  • MGC88685 antibody
  • OCAS p38 component antibody
  • OCT1 coactivator in S phase 38 KD Component antibody
  • Peptidyl-cysteine S-nitrosylase GAPDH antibody
see all

Anti-GAPDH antibody images:

  Western blot - GAPDH antibody (ab15822)

Western blot - GAPDH antibody (ab15822)

Anti-GAPDH antibody (ab15822) at 1 µg/ml + HeLa whole cell lysate at 20 µg

Secondary
Goat Anti-Chicken IgG (H&L) secondary at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 40 kDa


ab15822 detects a band of ~ 40 kDa in HeLa, Jurkat and A431 whole cell lysates.

See Abreview

  Immunocytochemistry/ Immunofluorescence - GAPDH antibody (ab15822)

Immunocytochemistry/ Immunofluorescence - GAPDH antibody (ab15822)

ICC/IF image of ab15822 stained human MCF7 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab15822, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-chicken IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and HepG2 cells.

References for Anti-GAPDH antibody (ab15822)

ab15822 has not yet been referenced specifically in any publications.

Publishing research using ab15822? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"