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Synthetic peptide: DAPMFVMGVNHFKYA , corresponding to amino acids 126-140 of Human GAPDH.
Our Abpromise guarantee covers the use of ab22555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 0.1 µg/ml. Detects a band of approximately 36 kDa.|
This image is courtesy of an anonymous Abreview
All Lanes: ab22555 (1/15000) staining GAPDH in Mouse brain whole tissue lysate (50 µg).
Blocking Step: 5% Milk for 1 hour at 20°C.
Secondary Antibody: HRP-conjugated Goat anti-rabbit IgG polyclonal (1/30000).
ab22555 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab22555 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"