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- Secondary antibodies
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Product name
Anti-GAPDH antibody
See all GAPDH products (26) ...
Description
Rabbit polyclonal to GAPDH
Tested applications
Cross reactivity
Reacts with
Rat, Human
Predicted to work with
Mouse, Rabbit, Chicken, Cow, Cat, Dog, Pig, Chinese Hamster
Immunogen
Synthetic peptide: VKVGVDGFGRIGRLV, corresponding to amino acids 3-17 of Human GAPDH.
VKVGVDGFGRIGRLV
Positive control
Rat heart protein extract.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: PBS, 1mg/ml BSA
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Types of disease >> Neurodegenerative disease
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Carbohydrate metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Energy Metabolism
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Huntington's disease
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Western blot - GAPDH antibody (ab22556)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab22556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa.
Target
Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Sequence similarities
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
Post-translational
modifications
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated.
Cellular localization
Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
Target information above from: UniProt accessionP04406
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
- Aging-associated gene 9 protein antibody
- BARS-38 antibody
see all
Database links
- Entrez Gene: 493876 Cat
- Entrez Gene: 374193 Chicken
- Entrez Gene: 281181 Cow
- Entrez Gene: 403755 Dog
- Entrez Gene: 2597 Human
- Entrez Gene: 100042025 Mouse
- Entrez Gene: 14433 Mouse
- Entrez Gene: 100135863 Rabbit
see all
Anti-GAPDH antibody images:
Western blot - GAPDH antibody (ab22556)
Anti-GAPDH antibody (ab22556) at 1 µg/ml
Performed under reducing conditions.
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
References for Anti-GAPDH antibody (ab22556)
This product has been referenced in:
- Goel M et al. Cochlin Induced TREK-1 Co-Expression and Annexin A2 Secretion: Role in Trabecular Meshwork Cell Elongation and Motility. PLoS One 6:e23070 (2011). WB; Human.Read more (PubMed: 21886777) »
- Hahn CK et al. Proteomic and genetic approaches identify Syk as an AML target. Cancer Cell 16:281-94 (2009). WB; Human.Read more (PubMed: 19800574) »
See all 3 publications for this product
Publishing research using ab22556? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"