Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Huntington's disease
Overview
Product name
Anti-GAPDH antibody
See all GAPDH products (9) ...
Description
Goat polyclonal to GAPDH
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Full length native protein from human erythrocytes.
Positive control
This antibody gave a positive signal in the following whole cell lysates: HeLa; NIH3T3. This antibody also gave a positive signal in Human brain tissue lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Energy Metabolism
Isotype/Loading Controls >> Loading Controls >> GAPDH
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Huntington's disease
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Detects a band of approximately 37 kDa (predicted molecular weight: 35.8 kDa).
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Sequence similarities
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
Post-translational
modifications
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated.
Cellular localization
Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
Target information above from: UniProt accessionP04406
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Aging associated gene 9 protein antibody
- G3P_HUMAN antibody
- G3PD antibody
see all
Database links
- Entrez Gene: 2597 Human
- Entrez Gene: 100042025 Mouse
- Entrez Gene: 14433 Mouse
- Omim: 138400 Human
- SwissProt: P04406 Human
- SwissProt: P16858 Mouse
- Unigene: 544577 Human
- Unigene: 592355 Human
see all
Anti-GAPDH antibody images:
Western blot - GAPDH antibody (ab9483)
All lanes : Anti-GAPDH antibody (ab9483) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lysates/proteins at 20 µg per lane.
Secondary
Rabbit polyclonal to Goat IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 35.8 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - GAPDH antibody (ab9483)
ICC/IF image of ab9483 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9483, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 1µg/ml.
References for Anti-GAPDH antibody (ab9483)
This product has been referenced in:
- Vorster PJet al. LIM kinase 1 modulates cortical actin and CXCR4 cycling and is activated by HIV-1 to initiate viral infection. J Biol Chem 286:12554-64 (2011).Read more (PubMed: 21321123) »
- Wilmer MJet al. Novel conditionally immortalized human proximal tubule cell line expressing functional influx and efflux transporters. Cell Tissue Res 339:449-57 (2010). WB; Human.Read more (PubMed: 19902259) »
See all 8 publications for this product
Publishing research using ab9483? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"