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Recombinant protein fragment corresponding to a region within amino acids 1 and 323 of Human GAPDH (NP_002037).
Our Abpromise guarantee covers the use of ab97626 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 36 kDa.|
|ICC/IF||1/100 - 1/200.|
|IP||Use a concentration of 5 µg/ml.|
GAPDH was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to GAPDH and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab97626.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 36kDa; GAPDH
ab97626 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"