Overview

  • Product nameAnti-GAPDH antibodySee all GAPDH primary antibodies ...
  • Description
    Rabbit polyclonal to GAPDH
  • Tested applicationsWB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 and 323 of Human GAPDH (NP_002037).

  • Positive control
    • 293T, A431, H1299, A549, HeLa S3, HepG2, MOLT4 and Raji cells

Properties

Applications

Our Abpromise guarantee covers the use of ab97626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 36 kDa.
ICC/IF 1/100 - 1/200.
IP Use a concentration of 5 µg/ml.

Target

Anti-GAPDH antibody images

  • GAPDH was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to GAPDH and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab97626.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 36kDa; GAPDH

  • All lanes : Anti-GAPDH antibody (ab97626) at 1/5000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate
    Lane 4 : Hela whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 36 kDa
    10% SDS PAGE
  • ab97626, at a 1/200 dilution, staining GAPDH in paraformaldehyde fixed A549. Lower image was merged with DNA probe.

References for Anti-GAPDH antibody (ab97626)

ab97626 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"