Anti-GAPDH antibody (ab97626)

Overview

• Product nameAnti-GAPDH antibodySee all GAPDH primary antibodies ...
• Description
  Rabbit polyclonal to GAPDH
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Cow, Cat, Dog, Pig
• Immunogen

  Recombinant protein fragment corresponding to a region within amino acids 1 and 323 of Human GAPDH (NP_002037).

• Positive control293T, A431, H1299, A549, HeLa S3, HepG2, MOLT4 and Raji cells

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
  Constituents: 20% Glycerol, 1% BSA, 1X PBS, pH 7
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Carbohydrate metabolism

  • Metabolism
  • Types of disease
  • Neurodegenerative disease

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of carbohydrates

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Other

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Huntington's disease

Applications

Target

• FunctionHas both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
• PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
• Sequence similaritiesBelongs to the glyceraldehyde-3-phosphate dehydrogenase family.
• Post-translational
  modificationsS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
  ISGylated.
• Cellular localizationCytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.

Target information above from: UniProt accession P04406 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 493876 Cat
  • Entrez Gene: 281181 Cow
  • Entrez Gene: 403755 Dog
  • Entrez Gene: 2597 Human
  • Entrez Gene: 100042025 Mouse
  • Entrez Gene: 14433 Mouse
  • Entrez Gene: 396823 Pig
  • Entrez Gene: 100009074 Rabbit

  • Entrez Gene: 24383 Rat
  • Entrez Gene: 685186 Rat
  • Omim: 138400 Human
  • SwissProt: P10096 Cow
  • SwissProt: Q28259 Dog
  • SwissProt: P04406 Human
  • SwissProt: P16858 Mouse
  • SwissProt: P00355 Pig
  • SwissProt: P46406 Rabbit
  • SwissProt: P04797 Rat
  • Unigene: 544577 Human
  • Unigene: 592355 Human
  • Unigene: 598320 Human
  • Unigene: 304088 Mouse
  • Unigene: 309092 Mouse
  • Unigene: 317779 Mouse
  • Unigene: 343110 Mouse
  • Unigene: 392463 Mouse
  • Unigene: 392480 Mouse
  • Unigene: 414470 Mouse
  • Unigene: 458138 Mouse
  • Unigene: 458416 Mouse
  • Unigene: 475698 Mouse
  • Unigene: 129558 Rat
  • Unigene: 91450 Rat

  see all

• Alternative names
  38 kDa BFA-dependent ADP-ribosylation substrate antibodyAging-associated gene 9 protein antibodyBARS-38 antibody

  cb609 antibodyEC 1.2.1.12 antibodyG3P_HUMAN antibodyG3PD antibodyG3PDH antibodyGAPD antibodyGAPDH antibodyGAPDH antibodyGlyceraldehyde 3 phosphate dehydrogenase antibodyGlyceraldehyde-3-phosphate dehydrogenase antibodyKNC-NDS6 antibodyMGC102544 antibodyMGC102546 antibodyMGC103190 antibodyMGC103191 antibodyMGC105239 antibodyMGC127711 antibodyMGC88685 antibodyOCAS, p38 component antibodyOCT1 coactivator in S phase, 38-KD component antibodyOTTHUMP00000174431 antibodyOTTHUMP00000174432 antibodyPeptidyl-cysteine S-nitrosylase GAPDH antibodywu:fb33a10 antibody

  see all

Anti-GAPDH antibody images

• 

  Western blot - GAPDH antibody (ab97626)
  All lanes : Anti-GAPDH antibody (ab97626) at 1/5000 dilution
  
  Lane 1 : 293T whole cell lysate
  Lane 2 : A431 whole cell lysate
  Lane 3 : H1299 whole cell lysate
  Lane 4 : Hela whole cell lysate
  
  Lysates/proteins at 30 µg per lane.
  
  
  Predicted band size : 36 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - GAPDH antibody (ab97626)
  ab97626, at a 1/200 dilution, staining GAPDH in paraformaldehyde fixed A549. Lower image was merged with DNA probe.