Anti-GAPDH antibody (ab97627)

Overview

• Product nameAnti-GAPDH antibodySee all GAPDH primary antibodies ...
• Description
  Rabbit polyclonal to GAPDH
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide corresponding to a region within amino acids 134 and 145 (VNHEKYDNSLK) of Human GAPDH (NP_002037)

  Run BLAST with Run BLAST with
• Positive controlA431, H1299, HeLa and HepG2 cell lines

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
  Constituents: 20% Glycerol, 1% BSA, 1X PBS, pH 7
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Neurodegenerative disease

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Carbohydrate metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Metabolism
  • Types of disease
  • Cancer

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of carbohydrates

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Other

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Huntington's disease

Applications

Target

• FunctionHas both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
• PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
• Sequence similaritiesBelongs to the glyceraldehyde-3-phosphate dehydrogenase family.
• Post-translational
  modificationsS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
  ISGylated.
• Cellular localizationCytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.

Target information above from: UniProt accession P04406 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2597 Human
  • Omim: 138400 Human
  • SwissProt: P04406 Human
  • Unigene: 544577 Human
  • Unigene: 592355 Human
  • Unigene: 598320 Human

• Alternative names
  38 kDa BFA-dependent ADP-ribosylation substrate antibodyAging-associated gene 9 protein antibodyBARS-38 antibody

  cb609 antibodyEC 1.2.1.12 antibodyG3P_HUMAN antibodyG3PD antibodyG3PDH antibodyGAPD antibodyGAPDH antibodyGAPDH antibodyGlyceraldehyde 3 phosphate dehydrogenase antibodyGlyceraldehyde-3-phosphate dehydrogenase antibodyKNC-NDS6 antibodyMGC102544 antibodyMGC102546 antibodyMGC103190 antibodyMGC103191 antibodyMGC105239 antibodyMGC127711 antibodyMGC88685 antibodyOCAS, p38 component antibodyOCT1 coactivator in S phase, 38-KD component antibodyPeptidyl-cysteine S-nitrosylase GAPDH antibodywu:fb33a10 antibody

  see all

Anti-GAPDH antibody images

• 

  Western blot - GAPDH antibody (ab97627)
  Anti-GAPDH antibody (ab97627) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
  
  Predicted band size : 36 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody (ab97627)
  ab97627, at 1/100 dilution, staining GAPDH in paraformaldehyde fixed Hela. Lower image was co-stained with Hoechst 33342.