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Anti-GAPDH antibody [mAbcam 9484] (HRP) - Loading Control (ab9482)

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Overview

Product name

Anti-GAPDH antibody [mAbcam 9484] (HRP) - Loading Control
See all GAPDH products (9) ...

Description

Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (HRP)

Conjugation

HRP

Conjugation notes

This antibody has been conjugated with HRP. The concentration of the original antibody was 1 mg/ml, following conjugation the concentration observed will be higher.

Specificity

This antibody shows a single clean band in Western blotting on cell lysates.

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Pig, Xenopus laevis, Chinese Hamster

Immunogen

Full length native protein from human erythrocytes.

General notes

According to our customer's feedback this antibody does not recognise meningococcal GapA1 or GapA2 (GAPDH) recombinant proteins.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 50% Glycerol, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

mAbcam 9484

Myeloma

Sp2/0-Ag14

Isotype

IgG2b

Light chain type

kappa

Research areas

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Energy Metabolism
Isotype/Loading Controls >> Loading Controls >> GAPDH
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Huntington's disease

  • Western blot - GAPDH antibody [mAbcam 9484] (HRP) - Loading Control (ab9482)Western blot - GAPDH antibody [mAbcam 9484] (HRP) - Loading Control (ab9482) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab9482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide9 Images

    WB

    Five stars (14 Abreviews) WB: 1/5000 - 1/10000.Det...Read more →

    WB: 1/5000 - 1/10000.Detects a band of approximately 40 kDa (predicted molecular weight: 40.2 kDa).(NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this ab following concerning customer feedback on the lack of signal with some vials. We found that milk significantly decreases signal and is therefore not a suitable blocking agent for this ab (see images of our tests on the datasheet of the unconjugated version, ab9484). This change in characteristics of the ab is due to a recent update to the production process.)

Target

Function

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.

Sequence similarities

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Post-translational
modifications

S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated.

Cellular localization

Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.

Target information above from: UniProt accessionP04406 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Aging associated gene 9 protein antibody
  • G3P_HUMAN antibody
  • G3PD antibody
  • G3PD antibody
  • G3PDH antibody
  • GAPD antibody
  • GAPDH antibody
  • GAPDH antibody
  • Glyceraldehyde 3 phosphate dehydrogenase antibody
  • Glyceraldehyde 3 phosphate dehydrogenase liver antibody
  • Glyceraldehyde 3 phosphate dehydrogenase muscle antibody
  • Glyceraldehyde-3-phosphate dehydrogenase antibody
  • MGC88685 antibody
  • OCAS p38 component antibody
  • OCT1 coactivator in S phase 38 KD Component antibody
  • Peptidyl-cysteine S-nitrosylase GAPDH antibody
see all

Database links

see all

Anti-GAPDH antibody [mAbcam 9484] (HRP) - Loading Control images:

  Western blot - GAPDH antibody [mAbcam 9484] (HRP) - Loading Control (ab9482)

Western blot - GAPDH antibody [mAbcam 9484] (HRP) - Loading Control (ab9482)

Lanes 1 - 4 : Anti-GAPDH antibody [mAbcam 9484] (HRP) - Loading Control (ab9482) at 1/5000 dilution ((Blocked in 5% MILK))
Lanes 5 - 8 : Anti-GAPDH antibody [mAbcam 9484] (HRP) - Loading Control (ab9482) at 1/5000 dilution ((Blocked in 5% BSA))

Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 7 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 8 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 40.2 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)


Exposure time : 30 seconds

References for Anti-GAPDH antibody [mAbcam 9484] (HRP) - Loading Control (ab9482)

This product has been referenced in:

  • Cao Zet al. Proteomic analysis of chicken embryonic trachea and kidney tissues after infection in ovo by avian infectious bronchitis coronavirus. Proteome Sci 9:11 (2011). WB; Chicken.Read more (PubMed: 21385394) »
  • Gunn Aet al. Correct End Use during End Joining of Multiple Chromosomal Double Strand Breaks Is Influenced by Repair Protein RAD50, DNA-dependent Protein Kinase DNA-PKcs, and Transcription Context. J Biol Chem 286:42470-82 (2011). WB.Read more (PubMed: 22027841) »

See all 21 publications for this product

Publishing research using ab9482? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"