Anti-GAPDH antibody [mAbcam 9484] - Loading Control (Alexa Fluor® 680) (ab184095)

Overview

  • Product name
    Anti-GAPDH antibody [mAbcam 9484] - Loading Control (Alexa Fluor® 680)
    See all GAPDH primary antibodies
  • Description
    Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680)
  • Conjugation
    Alexa Fluor® 680. Ex: 679nm, Em: 702nm
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Positive control
    • This antibody gave a positive signal in HeLa Nuclear as well as the following whole cell lysates: A431; HEK293; Jurkat.
  • General notes

    According to our customer's feedback this antibody does not recognise meningococcal GapA1 or GapA2 (GAPDH) recombinant proteins.

    Alternative versions available:
    Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)
    Anti-GAPDH antibody (HRP) [mAbcam 9484] - Loading Control (ab9482)
    Anti-GAPDH antibody (Alexa Fluor® 790) [mAbcam 9484] (ab184578)
    Anti-GAPDH antibody (Alexa Fluor® 647)  [mAbcam 9484] (ab196697)

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com

Properties

Applications

Our Abpromise guarantee covers the use of ab184095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).

Target

  • Function
    Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
  • Pathway
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
  • Sequence similarities
    Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
  • Post-translational
    modifications
    S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
    ISGylated.
  • Cellular localization
    Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aging associated gene 9 protein antibody
    • Epididymis secretory sperm binding protein Li 162eP antibody
    • G3P_HUMAN antibody
    • G3PD antibody
    • G3PDH antibody
    • GAPD antibody
    • GAPDH antibody
    • Glyceraldehyde 3 phosphate dehydrogenase antibody
    • Glyceraldehyde-3-phosphate dehydrogenase antibody
    • HEL S 162eP antibody
    • Peptidyl-cysteine S-nitrosylase GAPDH antibody
    see all

Anti-GAPDH antibody [mAbcam 9484] - Loading Control (Alexa Fluor® 680) images

  • All lanes : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (Alexa Fluor® 680) (ab184095) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 37 kDa
    Observed band size : 37 kDa

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab184095 overnight at 4°C. Antibody binding was detected after washing to remove excess antibody and imaged using the Licor Odyssey CLx.

References for Anti-GAPDH antibody [mAbcam 9484] - Loading Control (Alexa Fluor® 680) (ab184095)

ab184095 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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